ReadiCleave™ ROXtra AML-NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML ROXtra contains an azidomethyl linker that can be cleaved with TCEP to remove the ROXtra fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. ROXtra is the newly developed fluorophore that has the same spectra to the standard ROX dye with significantly improve stability and water solubility.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
7002	1 mg	Price

References

View all 50 references: Citation Explorer

Development of a PCR-lateral flow assay for rapid detection of Yersinia pestis, the causative agent of plague: PCR-LF assay for Yersinia pestis.

Authors:

Singh, Rita and Pal, Vijai and Kumar, Manoj and Tripathi, N K and Goel, A K

Journal:

Acta tropica (2021): 105958

Testing of four-sample pools offers resource optimization without compromising diagnostic performance of real time reverse transcriptase-PCR assay for COVID-19.

Authors:

Singh, Anirudh K and Nema, Ram Kumar and Joshi, Ankur and Shankar, Prem and Gupta, Sudheer and Yadav, Ashvini Kumar and Nema, Shashwati and Mathew, Bijina J and Shrivas, Arti and Patankar, Chitra and Raghuwanshi, Arun and Pandey, Ritu and Tripathi, Ranu and Ansari, Kudsia and Singh, Kuldeep and Yadav, Jogender and Biswas, Debasis and Singh, Sarman

Journal:

PloS one (2021): e0251891

Disruptors, a new class of oligonucleotide reagents, significantly improved PCR performance on templates containing stable intramolecular secondary structures.

Authors:

Liu, Zhaocheng and Zhao, Chengxue and Zhao, Guodong and Xiong, Shangmin and Ma, Yong and Zheng, Minxue

Journal:

Analytical biochemistry (2021): 114169

Laboratory Diagnosis of 37 Cases of Bartonella Endocarditis Based on Enzyme Immunoassay and Real-Time PCR.

Authors:

Shapira, Lev and Rasis, Michal and Binsky Ehrenreich, Inbal and Maor, Yasmin and Katchman, Eugene A and Treves, Adi and Velan, Ariel and Halutz, Ora and Graidy-Varon, Merav and Leibovitch, Cecilia and Maisler, Noam and Ephros, Moshe and Giladi, Michael

Journal:

Journal of clinical microbiology (2021)

Real-time PCR-based Y-specific sperm quantification assay in Queensland fruit fly: Insights to patterns of sperm storage.

Authors:

Shadmany, J and Lee, S F and Taylor, P W

Journal:

Insect molecular biology (2021): 315-324

Physical properties

Molecular weight	1379.58
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 27, 2025