Name: Protonex™ Red 780 acid
Brand: AAT Bioquest
SKU: 21185
Price: 203 USD
Availability: InStock

Protonex™ Red 780 acid

Protonex™ Red 780 works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 780 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 780 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 780 to become highly fluorescent and emit near-infrared light when excited by a red laser. By measuring the fluorescence intensity of Protonex™ Red 780, one can monitor the activation and trafficking of receptors or antibodies in live cells. Protonex™ Red 780 is especially useful in studying the activation and trafficking of G protein-coupled receptors (GPCRs), one of the most popular therapeutic drug targets. Protonex™ Red 780 can be used to label any receptor or epitope tag antibody and monitor its movement from the cell surface into acidic endosomes upon agonist stimulation. Protonex™ Red 780 might also be used to measure high-potency agonist and antagonist responses of different GPCRs in live cells.

The pH-dependent absorbance spectra of Protonex™ Red 780.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21185	1 mg	Price

References

View all 50 references: Citation Explorer

Generalization of the Ratiometric Method to Extend pH Range Measurements of the BCECF Probe.

Authors:

Tafech, Alaa and Beaujean, Céline and Usson, Yves and Stéphanou, Angélique

Journal:

Biomolecules (2023)

mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.

Authors:

Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark

Journal:

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49

Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome.

Authors:

Kashirina, Alena and Gavrina, Alena and Kryukov, Emil and Elagin, Vadim and Kolesova, Yuliya and Artyuhov, Alexander and Momotyuk, Ekaterina and Abdyyev, Vepa and Meshcheryakova, Natalia and Zagaynova, Elena and Dashinimaev, Erdem and Kashina, Aleksandra

Journal:

Biomedicines (2021)

Plastidial transporters KEA1 and KEA2 at the inner envelope membrane adjust stromal pH in the dark.

Authors:

Aranda Sicilia, María Nieves and Sánchez Romero, María Elena and Rodríguez Rosales, María Pilar and Venema, Kees

Journal:

The New phytologist (2021): 2080-2090

Effects of Diluent pH on Enrichment and Performance of Dairy Goat X/Y Sperm.

Authors:

He, Qifu and Wu, Shenghui and Huang, Ming and Wang, Ying and Zhang, Kang and Kang, Jian and Zhang, Yong and Quan, Fusheng

Journal:

Frontiers in cell and developmental biology (2021): 747722

Physical properties

Molecular weight	857.18
Solvent	DMSO

Spectral properties

Excitation (nm)	748
Emission (nm)	769

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 14, 2025