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Protonex™ Red 670 acid

Protonex™ Red 670 works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 670 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 670 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 670 to become highly fluorescent and emit red light when excited by a red laser such as a 632 nm He-Ne or 647 nm red laser. By measuring the fluorescence intensity of Protonex™ Red 670, one can monitor the activation and trafficking of receptors or antibodies in live cells. Protonex™ Red 670 is especially useful in studying the activation and trafficking of G protein-coupled receptors (GPCRs), one of the most popular therapeutic drug targets. Protonex™ Red 670 can be used to label any receptor or epitope tag antibody and monitor its movement from the cell surface into acidic endosomes upon agonist stimulation. Protonex™ Red 670 can also be used to measure high-potency agonist and antagonist responses of different GPCRs in live cells, such as the activation of TRHR-1 and beta-adrenoceptor, two GPCRs that are involved in hormone regulation and cardiovascular function.

Spectrum

Product family

References

View all 50 references: Citation Explorer
Mechanisms of 15-Epi-LXA4-Mediated HO-1 in Cytoprotection Following Inflammatory Injury.
Authors: Wang, Meng and Tong, Kun and Chen, Zhe and Wen, Zhengde
Journal: The Journal of surgical research (2023): 245-255
Generalization of the Ratiometric Method to Extend pH Range Measurements of the BCECF Probe.
Authors: Tafech, Alaa and Beaujean, Céline and Usson, Yves and Stéphanou, Angélique
Journal: Biomolecules (2023)
The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach.
Authors: Gradogna, Antonella and Pardo, José M and Carpaneto, Armando
Journal: Biomolecular concepts (2022): 119-125
FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells.
Authors: Kashirina, Alena and Gavrina, Alena and Mozherov, Artem and Kozlov, Dmitriy and Kuznetsova, Daria and Vorotelyak, Ekaterina and Zagaynova, Elena and Kalabusheva, Ekaterina and Kashina, Aleksandra
Journal: Cells (2022)
mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.
Authors: Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
Page updated on December 17, 2024

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Catalog Number21181
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Physical properties

Molecular weight

629.76

Solvent

DMSO

Spectral properties

Excitation (nm)

643

Emission (nm)

660

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Fluorescence response of Protonex™ Red 670 in various pH solutions.
Fluorescence response of Protonex™ Red 670 in various pH solutions.
Fluorescence response of Protonex™ Red 670 in various pH solutions.
The pH-dependent absorbance spectra of Protonex™ Red 670.
The pH-dependent Emission spectra of Protonex™ Red 670.