Protonex™ Red 670 acid
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 629.76 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 643 |
Emission (nm) | 660 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Intracellular pH
Molecular weight 629.76 | Excitation (nm) 643 | Emission (nm) 660 |
Protonex™ Red 670 works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 670 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 670 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 670 to become highly fluorescent and emit red light when excited by a red laser such as a 632 nm He-Ne or 647 nm red laser. By measuring the fluorescence intensity of Protonex™ Red 670, one can monitor the activation and trafficking of receptors or antibodies in live cells. Protonex™ Red 670 is especially useful in studying the activation and trafficking of G protein-coupled receptors (GPCRs), one of the most popular therapeutic drug targets. Protonex™ Red 670 can be used to label any receptor or epitope tag antibody and monitor its movement from the cell surface into acidic endosomes upon agonist stimulation. Protonex™ Red 670 can also be used to measure high-potency agonist and antagonist responses of different GPCRs in live cells, such as the activation of TRHR-1 and beta-adrenoceptor, two GPCRs that are involved in hormone regulation and cardiovascular function.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 670 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 158.791 µL | 793.953 µL | 1.588 mL | 7.94 mL | 15.879 mL |
5 mM | 31.758 µL | 158.791 µL | 317.581 µL | 1.588 mL | 3.176 mL |
10 mM | 15.879 µL | 79.395 µL | 158.791 µL | 793.953 µL | 1.588 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) |
Protonex™ Red 670 AM *Cell-Permeable* | 643 | 660 |
Protonex™ Red 780 acid | 748 | 769 |
Protonex™ Lyso-Red 670 | 643 | 660 |
Images
References
View all 50 references: Citation Explorer
Mechanisms of 15-Epi-LXA4-Mediated HO-1 in Cytoprotection Following Inflammatory Injury.
Authors: Wang, Meng and Tong, Kun and Chen, Zhe and Wen, Zhengde
Journal: The Journal of surgical research (2023): 245-255
Authors: Wang, Meng and Tong, Kun and Chen, Zhe and Wen, Zhengde
Journal: The Journal of surgical research (2023): 245-255
Generalization of the Ratiometric Method to Extend pH Range Measurements of the BCECF Probe.
Authors: Tafech, Alaa and Beaujean, Céline and Usson, Yves and Stéphanou, Angélique
Journal: Biomolecules (2023)
Authors: Tafech, Alaa and Beaujean, Céline and Usson, Yves and Stéphanou, Angélique
Journal: Biomolecules (2023)
The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach.
Authors: Gradogna, Antonella and Pardo, José M and Carpaneto, Armando
Journal: Biomolecular concepts (2022): 119-125
Authors: Gradogna, Antonella and Pardo, José M and Carpaneto, Armando
Journal: Biomolecular concepts (2022): 119-125
FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells.
Authors: Kashirina, Alena and Gavrina, Alena and Mozherov, Artem and Kozlov, Dmitriy and Kuznetsova, Daria and Vorotelyak, Ekaterina and Zagaynova, Elena and Kalabusheva, Ekaterina and Kashina, Aleksandra
Journal: Cells (2022)
Authors: Kashirina, Alena and Gavrina, Alena and Mozherov, Artem and Kozlov, Dmitriy and Kuznetsova, Daria and Vorotelyak, Ekaterina and Zagaynova, Elena and Kalabusheva, Ekaterina and Kashina, Aleksandra
Journal: Cells (2022)
mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.
Authors: Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
Authors: Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
RNA-seq analysis reveals genes related to photosynthetic carbon partitioning and lipid production in Phaeodactylum tricornutum under alkaline conditions.
Authors: Liu, Jian and Yin, Weihua and Zhang, Xinya and Xie, Xuan and Dong, Guanghui and Lu, Yao and Tao, Baoxiang and Gong, Qiangbin and Chen, Xinyan and Shi, Chao and Qin, Yuan and Zeng, Rensen and Li, Dawei and Li, Hongye and Zhao, Chao and Zhang, Huiying
Journal: Frontiers in microbiology (2022): 969639
Authors: Liu, Jian and Yin, Weihua and Zhang, Xinya and Xie, Xuan and Dong, Guanghui and Lu, Yao and Tao, Baoxiang and Gong, Qiangbin and Chen, Xinyan and Shi, Chao and Qin, Yuan and Zeng, Rensen and Li, Dawei and Li, Hongye and Zhao, Chao and Zhang, Huiying
Journal: Frontiers in microbiology (2022): 969639
ClC-K2 Cl- channel allows identification of A- and B-type of intercalated cells in split-opened collecting ducts.
Authors: Pyrshev, Kyrylo and Khayyat, Naghmeh Hassanzadeh and Stavniichuk, Anna and Tomilin, Viktor N and Zaika, Oleg and Ramkumar, Nirupama and Pochynyuk, Oleh
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022): e22275
Authors: Pyrshev, Kyrylo and Khayyat, Naghmeh Hassanzadeh and Stavniichuk, Anna and Tomilin, Viktor N and Zaika, Oleg and Ramkumar, Nirupama and Pochynyuk, Oleh
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022): e22275
Biochemical properties of H+-Ca2+-exchanger in the myometrium mitochondria.
Authors: Danylovych, Yurii V and Danylovych, Hanna V and Kolomiets, Oksana V and Sviatnenko, Marina D and Kosterin, Sergiy O
Journal: Current research in physiology (2022): 369-380
Authors: Danylovych, Yurii V and Danylovych, Hanna V and Kolomiets, Oksana V and Sviatnenko, Marina D and Kosterin, Sergiy O
Journal: Current research in physiology (2022): 369-380
Increased sarcolemma chloride conductance as one of the mechanisms of action of carbonic anhydrase inhibitors in muscle excitability disorders.
Authors: Altamura, Concetta and Fonzino, Adriano and Tarantino, Nancy and Conte, Elena and Liantonio, Antonella and Imbrici, Paola and Carratù, Maria Rosaria and Pierno, Sabata and Desaphy, Jean-François
Journal: Experimental neurology (2021): 113758
Authors: Altamura, Concetta and Fonzino, Adriano and Tarantino, Nancy and Conte, Elena and Liantonio, Antonella and Imbrici, Paola and Carratù, Maria Rosaria and Pierno, Sabata and Desaphy, Jean-François
Journal: Experimental neurology (2021): 113758
Glomerular Mesangial Cell pH Homeostasis Mediates Mineralocorticoid Receptor-Induced Cell Proliferation.
Authors: Gekle, Michael and Mildenberger, Sigrid
Journal: Biomedicines (2021)
Authors: Gekle, Michael and Mildenberger, Sigrid
Journal: Biomedicines (2021)
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Cell Preparation Considerations and Troubleshooting
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
Cell Preparation Considerations and Troubleshooting
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What are the advantages of a Nanodrop light spectrophotometer?
What are the disadvantages of Nanodrop light spectrophotometer?
What is an AM ester?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What are the advantages of a Nanodrop light spectrophotometer?
What are the disadvantages of Nanodrop light spectrophotometer?
What is an AM ester?