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AAT Bioquest

Phalloidin Conjugates

XFD594 Same Structure to Alexa Fluor™ 594
XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher). XFD594 phalloidin conjugate is chemically equivalent to Alexa Fluor® 594 phalloidin. This red fluorescent phalloidin conjugate selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.6610.430.56
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.250.19
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.30.11
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*6506712390000.3310.000.03
XFD594 acid590618900000.6610.430.56
XFD594 azide590618900000.6610.430.56
XFD594 alkyne590618900000.6610.430.56
XFD594 TCO590618900000.6610.430.56
XFD594 Tetrazine590618900000.6610.430.56

Citations

View all 28 citations: Citation Explorer
Effective Orthodontic Tooth Movement via an Occlusion-Activated Electromechanical Synergistic Dental Aligner
Authors: Wang, Qian and Zhang, Jie and Yao, Guang and Lou, Wenhao and Zhang, Tianyao and Zhang, Zihan and Xie, Maowen and Gan, Xingyi and Pan, Taisong and Gao, Min and others,
Journal: ACS nano (2023)
Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression
Authors: Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2017): 8
DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes
Authors: Sun, Ming-Hong and Yang, Mo and Xie, Feng-Yun and Wang, Wei and Zhang, Lili and Shen, Wei and Yin, Shen and Ma, Jun-Yu
Journal: PloS one (2017): e0170308
Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization
Authors: Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung
Journal: ACS Applied Materials & Interfaces (2017)
Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery
Authors: Chang, Ting-Ya and Chen, Chen and Lee, Min and Chang, Ya-Chu and Lu, Chi-Huan and Lu, Shao-Tzu and Wang, De-Yao and Wang, Aijun and Guo, Chin-Lin and Cheng, Pei-Lin
Journal: eLife (2017): e31101

References

View all 127 references: Citation Explorer
Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes
Authors: Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.
Journal: Urology (2011): 970 e1
Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin
Authors: Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.
Journal: FEBS Lett (2011): 767
Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging
Authors: Chazotte B., undefined
Journal: Cold Spring Harb Protoc (2010): pdb prot4947
pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation
Authors: An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.
Journal: Proc Natl Acad Sci U S A (2010): 20246
Protective effect of bile acid derivatives in phalloidin-induced rat liver toxicity
Authors: Herraez E, Macias RI, Vazquez-Tato J, Hierro C, Monte MJ, Marin JJ.
Journal: Toxicol Appl Pharmacol (2009): 21
Page updated on October 9, 2024

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Physical properties

Molecular weight

~1600

Solvent

DMSO

Spectral properties

Absorbance (nm)

592

Correction Factor (260 nm)

0.43

Correction Factor (280 nm)

0.56

Extinction coefficient (cm -1 M -1)

92000

Excitation (nm)

590

Emission (nm)

618

Quantum yield

0.661

Storage, safety and handling

H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
<strong>Fixed and stained HeLa cells.</strong><br>HeLa cells were fixed with 4% formaldehyde, permeabilized, and blocked. F-actin were stained with XFD594 phalloidin (Cat No. 23158) and nuclei labeled with DAPI (Cat No. 17507). Images were acquired on a Keyence BZ-X710 all-in-one fluorescence microscope.
<strong>Fixed and stained HeLa cells.</strong><br>HeLa cells were fixed with 4% formaldehyde, permeabilized, and blocked. F-actin were stained with XFD594 phalloidin (Cat No. 23158) and nuclei labeled with DAPI (Cat No. 17507). Images were acquired on a Keyence BZ-X710 all-in-one fluorescence microscope.
<strong>Fixed and stained HeLa cells.</strong><br>HeLa cells were fixed with 4% formaldehyde, permeabilized, and blocked. F-actin were stained with XFD594 phalloidin (Cat No. 23158) and nuclei labeled with DAPI (Cat No. 17507). Images were acquired on a Keyence BZ-X710 all-in-one fluorescence microscope.