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Oligo(dT) Magnetic Beads
Optimized for mRNA Extraction, 1.0%
Oligo(dT)30 magnetic beads extract and isolate mRNA from crude samples, cell lysates or tissues, by hybridizing with the poly(A) tail. Captured mRNA is available for downstream applications such as cDNA synthesis and RT-PCR.
Given the growing interest in transcriptome studies, it has become essential to be able to isolate mRNA against related species, such as transfer RNA (tRNA) and ribosomal RNA (rRNA). Many RNA purification techniques, however, are non-specific to mRNA. Additionally, mRNA only constitutes a small portion of total cellular RNA, further making purification difficult. Fortunately, by targeting the polyadenylated RNA tail on the 3’ end of mRNA, oligo(dT)-coated magnetic beads can easily extract mRNA under standard hybridization conditions. Once extracted, bound mRNA can conveniently be eluted or serve directly as the primer for cDNA synthesis with reverse transcriptase.
| Density | 1.4 g/cm3 |
| Diameter | 0.7 µm |
| Bead concentration | 1% (10 mg / mL) |
| Storage buffer | PBS pH 7.4, 0.1% Proclin 300 |
| Binding capacity | 10 µg mRNA per 100 µL (1 mg) of magnetic beads solution |
References
View all 13 references: Citation Explorer
Development of a 3D-printed single-use separation chamber for use in mRNA-based vaccine production with magnetic microparticles.
Authors: Wommer, Lars and Meiers, Patrick and Kockler, Isabelle and Ulber, Roland and Kampeis, Percy
Journal: Engineering in life sciences (2021): 573-588
Authors: Wommer, Lars and Meiers, Patrick and Kockler, Isabelle and Ulber, Roland and Kampeis, Percy
Journal: Engineering in life sciences (2021): 573-588
Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene.
Authors: Krejcova, Ludmila and Hynek, David and Kopel, Pavel and Rodrigo, Miguel Angel Merlos and Adam, Vojtech and Hubalek, Jaromir and Babula, Petr and Trnkova, Libuse and Kizek, Rene
Journal: Viruses (2013): 1719-39
Authors: Krejcova, Ludmila and Hynek, David and Kopel, Pavel and Rodrigo, Miguel Angel Merlos and Adam, Vojtech and Hubalek, Jaromir and Babula, Petr and Trnkova, Libuse and Kizek, Rene
Journal: Viruses (2013): 1719-39
Prognostic significance of survivin-expressing circulating tumour cells in T1G3 bladder cancer.
Authors: Gradilone, Angela and Petracca, Arianna and Nicolazzo, Chiara and Gianni, Walter and Cortesi, Enrico and Naso, Giuseppe and Vincenzi, Bruno and Cristini, Cristiano and De Berardinis, Ettore and Di Silverio, Franco and Aglianò, Anna Maria and Gazzaniga, Paola
Journal: BJU international (2010): 710-5
Authors: Gradilone, Angela and Petracca, Arianna and Nicolazzo, Chiara and Gianni, Walter and Cortesi, Enrico and Naso, Giuseppe and Vincenzi, Bruno and Cristini, Cristiano and De Berardinis, Ettore and Di Silverio, Franco and Aglianò, Anna Maria and Gazzaniga, Paola
Journal: BJU international (2010): 710-5
DNA hybridization at microbeads with cathodic stripping voltammetric detection.
Authors: Palecek, E and Billová, S and Havran, L and Kizek, R and Miculková, A and Jelen, F
Journal: Talanta (2002): 919-30
Authors: Palecek, E and Billová, S and Havran, L and Kizek, R and Miculková, A and Jelen, F
Journal: Talanta (2002): 919-30
[Subtractive gene cloning using dynabeads oligo(dT)25 for elucidation of pseudohyphal formation in Candida tropicalis].
Authors: Imanishi, Y and Kawai, T and Iwaguchi, S and Suzuki, T and Kamihara, T and Yokoyama, K and Nishimura, K
Journal: Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology (2001): 243-51
Authors: Imanishi, Y and Kawai, T and Iwaguchi, S and Suzuki, T and Kamihara, T and Yokoyama, K and Nishimura, K
Journal: Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology (2001): 243-51
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