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ReadiPrep™ Ni-TED Magnetic Agarose Beads
Ni-TED magnetic agarose beads streamline the purification of His-tagged proteins in a manner analogous to Ni-NTA.
This resin consists of magnetic agarose covalently attached to Ni-TED (Nickel - Tris(carboxymethyl)ethylene diamine). This allows the binding of recombinant proteins carrying the polyhistidine (6xHis) tag with high specificity and affinity. Once bound, other proteins can be washed away, after which His-tagged proteins can be recovered with an elution buffer.
Ni-TED magnetic agarose beads are provided as a 25% slurry. The binding capacity is greater than 10 mg of His-tagged protein per 1 mL of settled beads. High quality manufacturing protocols ensure minimal lot-to-lot variability, and the ready-to-use format satisfies both small and large scale purification needs. Compared with conventional purification methods, magnetic agarose beads do not require centrifugation, thereby reducing hands-on time.
References
View all 4 references: Citation Explorer
Heterologous Expression and Characterization of a Thermostable α-L-Rhamnosidase from Thermoclostridium stercorarium subsp. thermolacticum DSM 2910 and Its Application in the Biotransformation of Rutin.
Authors: Ge, Lin and Liu, Yingying and Zhou, Fangming and Zhan, Lingling and Zhao, Linguo
Journal: Journal of microbiology and biotechnology (2023): 1521-1530
Authors: Ge, Lin and Liu, Yingying and Zhou, Fangming and Zhan, Lingling and Zhao, Linguo
Journal: Journal of microbiology and biotechnology (2023): 1521-1530
The molecular adjuvant mC3d enhances the immunogenicity of FimA from type I fimbriae of Salmonella enterica serovar Enteritidis.
Authors: Musa, Hassan-Hussein and Zhang, Wei-Juan and Lv, Jing and Duan, Xiao-Li and Yang, Yang and Zhu, Chun-Hong and Li, Hui-Fang and Chen, Kuan-Wei and Meng, Xia and Zhu, Guo-Qiang
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2014): 57-62
Authors: Musa, Hassan-Hussein and Zhang, Wei-Juan and Lv, Jing and Duan, Xiao-Li and Yang, Yang and Zhu, Chun-Hong and Li, Hui-Fang and Chen, Kuan-Wei and Meng, Xia and Zhu, Guo-Qiang
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2014): 57-62
Site directed immobilization of glucose-6-phosphate dehydrogenase via thiol-disulfide interchange: influence on catalytic activity of cysteines introduced at different positions.
Authors: Simons, J R and Mosisch, M and Torda, A E and Hilterhaus, L
Journal: Journal of biotechnology (2013): 1-7
Authors: Simons, J R and Mosisch, M and Torda, A E and Hilterhaus, L
Journal: Journal of biotechnology (2013): 1-7
Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system.
Authors: Douillard, François P and O'Connell-Motherway, Mary and Cambillau, Christian and van Sinderen, Douwe
Journal: Microbial cell factories (2011): 66
Authors: Douillard, François P and O'Connell-Motherway, Mary and Cambillau, Christian and van Sinderen, Douwe
Journal: Microbial cell factories (2011): 66
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