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Product key features
- Effective Dead Cell Removal: Enrich live cell populations by removing dead cells with high efficiency.
- Versatile: Suitable for various sample types.
- High Purity: Targeting and removal of apoptotic and necrotic cells.
- Quick Protocol: Streamlined workflow with simple incubation steps and use of magnetic separation.
Product description
The ReadiPrep™ Dead Cell Removal Kit provides a fast and reliable method for enriching live cell populations by removing dead cells, including both apoptotic and necrotic cells, from your samples. This kit leverages Annexin V conjugation to specifically bind dead cells, followed by magnetic separation using magnetic spheres, ensuring that live cells are efficiently isolated from dead ones.
This kit is designed for ease of use with minimal hands-on time. The provided reagents and protocol can help to quickly obtain enriched live cell populations from a variety of sample types, such as cultured cells or tissue homogenates. The resulting live cell suspension is highly pure, making it ideal for downstream applications like flow cytometry, cell culture, or molecular analysis making it ideal for studies involving cell viability, apoptosis, or cellular responses to drug treatments.
Example protocol
AT A GLANCE
- Prepare cells in the Isolation Buffer (Component C).
- Add ReadiPrep™ Dead Cell (Annexin V) Conjugate (Component A).
- Incubate 10 min at RT.
- Add Magnetic Spheres (Component B).
- Incubate 10 min at RT.
- Place the tube in a magnetic rack.
- Wait for 2-3 min.
- Carefully collect the supernatant containing live cells.
SAMPLE EXPERIMENTAL PROTOCOL
- Prepare 1x10^7 cells in 500 µL Isolation Buffer (Component C).
- Add 25 µL ReadiPrep™ Dead Cell (Annexin V) Conjugate (Component A) to the sample. Incubate for 10 min at RT.
- Centrifuge the cells.
- Resuspend cells in 500 μL Isolation Buffer (Component C).
- Add 10 µL of Magnetic Spheres (Component B) to cell sample.
- Incubate for 10 min at RT. (The dead cells will bind to the Streptavidin Magnetic Spheres).
- Place the tube in the magnetic rack and incubate for 2 min.
- Collect the cell suspension into a new tube (the supernatant containing live cells).
| Cells number | Component A | Component B | Component C |
| 1X10^7 cells | 25 µL | 10 µL | 0.5 mL |
| 1X10^8 cells | 100 µL | 100 µL | 2 mL |
| 1X10^9 cells | 200 µL | 1 mL | 4 mL |
References
Authors: Johannisson, Anders and Morrell, Jane M and Wallgren, Margareta
Journal: Animal reproduction science (2024): 107493
Authors: Li, Weifeng and Gurdziel, Katherine and Pitchaikannu, Ahalya and Gupta, Naman and Hazlett, Linda D and Xu, Shunbin
Journal: The ocular surface (2023): 17-41
Authors: Li, Anqi and Barabadi, Mehri and McDonald, Hannah and Chan, Siow Teng and Krause, Mirja and Ooi, Joshua D and Kusuma, Gina D and James, David and Lim, Rebecca
Journal: Cytotherapy (2022): 650-658
Authors: Song, Qiaoling and Zhang, Yazhuo and Zhou, Mingming and Xu, Yuting and Zhang, Qianyue and Wu, Lihong and Liu, Shan and Zhang, Minghui and Zhang, Lei and Wu, Zhihua and Peng, Weixun and Liu, Xutao and Zhao, Chenyang
Journal: Frontiers in immunology (2022): 920232
Authors: Kwon, Taehong and Yao, Rujie and Hamel, Jean-François P and Han, Jongyoon
Journal: Lab on a chip (2018): 2826-2837
Product protocol