MitoDNA™ Red 710
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 342.91 |
Solvent | DMSO |
Excitation (nm) | 511 |
Emission (nm) | 707 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Molecular weight 342.91 | Excitation (nm) 511 | Emission (nm) 707 |
Platform
Fluorescence microscope
Excitation | 510 nm |
Emission | 710 nm |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Before using MitoDNA™ Red 710 for the first time, allow it to thaw at room temperature. Then, briefly centrifuge it to collect the dried pellet.
Prepare cells in a growth medium.
Stain cells with MitoDNA™ Red 710 working solution.
Incubate samples for 5 to 15 minutes at 37 °C.
Monitor fluorescence intensity at Ex/Em = 510/710 nm.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Prepare a 5 to 10 mM MitoDNA™ Red 710 stock solution in DMSO. For example, add 290 μL of DMSO to the MitoDNA™ Red 710 vial to create a 10 mM stock solution.
Note: Prepare a single aliquot of the unused MitoDNA™ Red 710 stock solution and store it at ≤ -20 º C, protected from light. Avoid repeated freeze-thaw cycles.
PREPARATION OF WORKING SOLUTION
Prepare a 5 to 10 μM working solution by diluting the MitoDNA™ Red 710 stock solution in Hanks' solution with 20 mM HEPES buffer (HHBS).
Note: For optimal results, use this solution within a few hours of preparation.
Note: Cover the working solution with foil or store it in a dark place to protect it from light.
SAMPLE EXPERIMENTAL PROTOCOL
Plate the cells in a 96-well plate with black walls and a clear bottom.
Remove the cell culture medium and add 100 µL of MitoDNA™ Red 710 working solution directly to the cells.
Incubate the cells at 37°C for 5-15 minutes, protected from light.
Note: The concentration and incubation time of MitoDNA™ Red 710 may vary depending on the cell line. Test different concentrations to determine the optimal dose.
Remove the dye working solution and wash the cells twice with HHBS buffer.
Add HHBS buffer and analyze the cells using a fluorescence microscope with excitation/emission settings of 510/710 nm.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 291.622 µL | 1.458 mL | 2.916 mL | 14.581 mL | 29.162 mL |
5 mM | 58.324 µL | 291.622 µL | 583.243 µL | 2.916 mL | 5.832 mL |
10 mM | 29.162 µL | 145.811 µL | 291.622 µL | 1.458 mL | 2.916 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
![The fluorescence response of MitoDNA™ Red 710 (5 µM) was assessed in HeLa cells before and after DNase treatment (2 units/reaction) at 37°C for 1 hour. Fluorescence intensities were measured using a fluorescence microscope equipped with a Violet long-pass filter (450 nm emission long-pass filter).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fmitodna-red-710%2Ffigure-for-mitodna-red-710_tv0dP.png&w=3840&q=75)
References
Authors: Li, Wenting and Li, Yuting and Zhao, Jie and Liao, Jiabao and Wen, Weibo and Chen, Yao and Cui, Huantian
Journal: Pathology, research and practice (2024): 155330
Authors: Huang, Penghui and Li, Li and Chen, Yaohua and Li, Yuping and Zhu, Dan and Cui, Jian
Journal: Open life sciences (2024): 20220872
Authors: Bulduk, Bengisu K and Tortajada, Juan and Valiente-Pallejà, Alba and Callado, Luís F and Torrell, Helena and Vilella, Elisabet and Meana, J Javier and Muntané, Gerard and Martorell, Lourdes
Journal: Psychiatry research (2024): 115928
Authors: Weissensteiner, Hansi and Forer, Lukas and Kronenberg, Florian and Schönherr, Sebastian
Journal: Nucleic acids research (2024)
Authors: Feng, Yue and You, Yingqian and Li, Mengying and Guan, Xin and Fu, Ming and Wang, Chenming and Xiao, Yang and He, Meian and Guo, Huan
Journal: The Science of the total environment (2024): 173767
Authors: Ferreira, Tomas and Rodriguez, Santiago
Journal: Genes (2024)
Authors: Fan, Xiaoteng and Zhang, Dingfu and Hou, Tingting and Zhang, Qianqing and Tao, Lu and Bian, Chongqian and Wang, Zaizhao
Journal: Environmental science & technology (2024): 7731-7742
Authors: Feng, Zhijun and Wang, Yinghui and Fu, Zhengzheng and Liao, Jing and Liu, Hui and Zhou, Meijuan
Journal: Nutrients (2024)
Authors:
Journal: Nature cell biology (2024)
Authors: Wang, Shuying and Tan, Jin and Zhang, Qiang
Journal: Neurochemical research (2024)
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