MitoDNA™ Red 610
Price | |
Catalog Number | |
Unit Size | |
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 344.93 |
Solvent | DMSO |
Excitation (nm) | 508 |
Emission (nm) | 607 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Molecular weight 344.93 | Excitation (nm) 508 | Emission (nm) 607 |
Platform
Fluorescence microscope
Excitation | 490 nm |
Emission | 610 nm |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Before using MitoDNA™ Red 610 for the first time, allow it to thaw at room temperature. Then, briefly centrifuge it to collect the dried pellet.
Prepare cells in a growth medium.
Stain cells with MitoDNA™ Red 610 working solution.
Incubate samples for 5 to 15 minutes at 37 °C.
Monitor fluorescence intensity at Ex/Em = 490/610 nm.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Prepare a 5 to 10 mM MitoDNA™ Red 610 stock solution in DMSO. For example, add 290 μL of DMSO to the MitoDNA™ Red 610 vial to create a 10 mM stock solution.
Note: Prepare a single aliquot of the unused MitoDNA™ Red 610 stock solution and store it at ≤ -20 º C, protected from light. Avoid repeated freeze-thaw cycles.
PREPARATION OF WORKING SOLUTION
Prepare a 5 to 10 μM working solution by diluting the MitoDNA™ Red 610 stock solution in Hanks' solution with 20 mM HEPES buffer (HHBS).
Note: For optimal results, use this solution within a few hours of preparation.
Note: Cover the working solution with foil or store it in a dark place to protect it from light.
SAMPLE EXPERIMENTAL PROTOCOL
Plate the cells in a 96-well plate with black walls and a clear bottom.
Remove the cell culture medium and add 100 µL of MitoDNA™ Red 610 working solution directly to the cells.
Incubate the cells at 37°C for 5-15 minutes, protected from light.
Note: The concentration and incubation time of MitoDNA™ Red 610 may vary depending on the cell line. Test different concentrations to determine the optimal dose.
Remove the dye working solution and wash the cells twice with HHBS buffer.
Add HHBS buffer and analyze the cells using a fluorescence microscope with excitation/emission settings of 490/610 nm.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 289.914 µL | 1.45 mL | 2.899 mL | 14.496 mL | 28.991 mL |
5 mM | 57.983 µL | 289.914 µL | 579.828 µL | 2.899 mL | 5.798 mL |
10 mM | 28.991 µL | 144.957 µL | 289.914 µL | 1.45 mL | 2.899 mL |
Molarity calculator
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Images
References
Authors: Bao, Yu and Yang, Shiyuan and Zhao, Hailan and Wang, Yezhen and Li, Ke and Liu, Xue and Zhang, Wei and Zhu, Xue
Journal: BMC pulmonary medicine (2024): 176
Authors: Zhang, Yingchi and Xu, Tao and Xie, Jie and Wu, Hua and Hu, Weihua and Yuan, Xuefeng
Journal: Cell communication and signaling : CCS (2024): 240
Authors: Dancis, Andrew and Pandey, Ashutosh K and Pain, Debkumar
Journal: Biochimica et biophysica acta. Molecular cell research (2024): 119733
Authors: Lei, Tang and Rui, Yu and Xiaoshuang, Zhou and Jinglan, Zhang and Jihong, Zhang
Journal: Cell death discovery (2024): 168
Authors: Feng, Fuhai and He, Shasha and Li, Xiaoling and He, Jiake and Luo, Lianxiang
Journal: Aging and disease (2024): 714-738
Authors: Sun, Chang and Zhao, Shanshan and Pan, Zimeng and Li, Jing and Wang, Yasong and Kuang, Hongying
Journal: DNA and cell biology (2024): 158-174
Authors: Rubilar, Juan Carlos and Outeiro, Tiago Fleming and Klein, Andrés D
Journal: Brain : a journal of neurology (2024)
Authors: Zong, Yuan and Yang, Yi and Zhao, Jiawen and Li, Lei and Luo, Danyang and Hu, Jiawei and Gao, Yiming and Xie, Xianfei and Shen, Linhui and Chen, Sheng and Ning, Li and Jiang, Liting
Journal: Computers in biology and medicine (2024): 108511
Authors: Ying, Lingxiao and Kong, Lin and Qiu, Xiaoxiao and Cheng, Aihua and Wang, Qijun and Xiu, Limeng and Shi, Jinmei and Tao, Yanfei and Chai, Zhihong
Journal: Journal of cellular and molecular medicine (2024): e18265
Authors: Sekula, Malgorzata and Tworzydlo, Waclaw and Bilinski, Szczepan M
Journal: Scientific reports (2024): 8263
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