Name: MitoDNA™ Red 680
Brand: AAT Bioquest
SKU: 22688
Price: 301 USD
Availability: InStock

MitoDNA™ Red 680

MitoDNA™ Red 680 is a far-red mitochondrial DNA probe with deep tissue penetration that enables selective mtDNA imaging in live cells and tissues with minimal autofluorescence background.

mtDNA-Specific: Selectively binds to mtDNA without nuclear interference
Live-Cell Compatibility: Easily permeates cells for real-time mtDNA tracking
High Signal Clarity: Large Stokes shift ensures a strong signal-to-noise ratio for multiplex imaging
Broad Research Applicability: Ideal for investigating mtDNA-linked diseases and mitochondrial function

The fluorescence response of MitoDNA™ Red 680 (5 µM) in HeLa cells was assessed before and after DNase treatment. Fluorescence intensities were monitored using fluorescence microscopy.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22688	1 mg	Price

Overview

There are limited probes available that effectively detect mitochondrial deoxyribonucleic acid (mtDNA). Common fluorescent DNA probes, such as DAPI, Hoechst, or SYBR® Green, lack the specificity required for mitochondrial targeting, primarily staining nuclear DNA. MitoDNA™ Red 680 is a cell-permeable dye that specifically stains mtDNA in live cells, offering an efficient method for the dynamic imaging of mtDNA. This dye exhibits a large Stokes shift, providing an excellent signal-to-noise ratio and enabling easy multiplex staining with other fluorescent probes. mtDNA is a small, circular DNA molecule located within mitochondria in the cytoplasm. It is supplementary to nuclear DNA and encodes 37 genes essential for mitochondrial and cellular functions. Mitochondria are responsible for ATP synthesis through oxidative phosphorylation and contain the genetic information for synthesizing key enzymes, transfer RNA (tRNA), and ribosomal RNA (rRNA). Mutations and disorders in mtDNA can lead to a range of health issues, including age-related hearing loss, diabetes, and failures in the brain, heart, and liver. Additionally, mtDNA mutations are associated with an increased risk of various cancers, including lymphomas, leukemias, and tumors in the breast, intestines, liver, and kidneys.

References

View all 50 references: Citation Explorer

Molecular and cellular consequences of mitochondrial DNA double-stranded breaks.

Authors:

Yu, Chenxiao and Asadian, Samieh and Tigano, Marco

Journal:

Human molecular genetics (2024): R12-R18

Coordinating mitochondrial translation with assembly of the OXPHOS complexes.

Authors:

Kremer, Laura S and Rehling, Peter

Journal:

Human molecular genetics (2024): R47-R52

Complete mitochondrial DNA sequence of Alboglossiphonia lata Oka, 1910 (Rhynchobdellida: Glossiphoniidae) and its phylogenetic analysis.

Authors:

Jin, Panpan and Tian, Yu and Zang, Erhuan and Zeng, Lingchao and Zhang, Zhaolei and Liu, Jinxin and Shi, Linchun

Journal:

Mitochondrial DNA. Part B, Resources (2024): 652-656

Exploring mitochondrial DNA copy number in circulating cell-free DNA and extracellular vesicles across cardiovascular health status: A prospective case-control pilot study.

Authors:

Rucci, Chiara and de Simone, Gaia and Salathia, Saniya and Casadidio, Cristina and Censi, Roberta and Bordoni, Laura

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2024): e23672

Mitochondrial DNA mutations in extremely preterm infants with bronchopulmonary dysplasia.

Authors:

Jeong, Jiyoon and Lee, Yeonmi and Han, Jongsuk and Kang, Eunju and Kim, Deokhoon and Kim, Ki-Soo and Kim, Ellen Ai-Rhan and Lee, Byong Sop and Jung, Euiseok

Journal:

Gene (2024): 148337

Physical properties

Molecular weight	486.44
Solvent	DMSO

Spectral properties

Excitation (nm)	597
Emission (nm)	681

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	600 nm
Emission	680 nm
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025