Fluorescein-12-dUTP 1 mM in TE Buffer (pH 7.5) CAS 214154-36-6

Name: Fluorescein-12-dUTP *1 mM in TE Buffer (pH 7.5)* *CAS 214154-36-6*
Brand: AAT Bioquest
SKU: 17022
Price: 112 USD
Availability: InStock

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-dUTP conjugate can be used as a green fluorescence color with Spectrum Green™ filter set (Spectrum Green™ is the trademark of Vysis).

Example protocol

AT A GLANCE

Important notes

Best stored at -80 °C, it can be diluted 10 folds in TE Buffer (10mM Tris-HCl + 1mM EDTA (pH=7.5)) for convenient pipetting. Expiration date is six months from the date of receipt.

SAMPLE EXPERIMENTAL PROTOCOL

Table:1. The following instruction is recommended as a starting point for labeling ~1µg dsDNA, optimum labeling conditions may vary for different cases.

Reagents	Final Concentration for 20µL reaction volume
DNA	0.05 µg/µL
DNA Polymerase	25 ~ 50U/mL
Fluorescein-dUTP	10 ~ 100 µM
Reaction Buffer	10 mM Tris-HCl (pH = 7.5) contains the following components: 1 mM EDTA 5 mM NaCl 0.1 mM DTT 1 mM dATP + 1 mM dCTP+ 1 mM dGTP+ 1 mM dTTP
ddH₂O	Adjust volume as need to make 20 µL reaction volume

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein-12-dCTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dATP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dGTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

References

View all 9 references: Citation Explorer

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Page updated on November 20, 2024

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Physical properties

Molecular weight	1060.62
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	214154-36-6