Fluorescein-12-dCTP 1 mM in Tris Buffer (pH 7.5)

Fluorescein-12-dCTP might be incorporated into a DNA sequence as its natural counterpart dCTP to incorporate a fluorescein tag in a DNA molecule. It can be used for the direct enzymatic labeling of DNA/cDNA, e.g., by PCR or Nick Translation. The resulting fluorescein-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. The linker between fluorescein tag and cytidine has been optimized for the optimal substrate incorporation and labeling efficiency.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein-12-dUTP 1 mM in TE Buffer (pH 7.5) CAS 214154-36-6	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dUTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dATP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dGTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

References

View all 18 references: Citation Explorer

Development and Preliminary Application of Multiplex Loop-Mediated Isothermal Amplification Coupled With Lateral Flow Biosensor for Detection of Mycobacterium tuberculosis Complex.
Authors: Wang, Xingyun and Wang, Guirong and Wang, Yacui and Quan, Shuting and Qi, Hui and Sun, Lin and Shen, Chen and Huang, Hairong and Jiao, Weiwei and Shen, Adong
Journal: Frontiers in cellular and infection microbiology (2021): 666492

Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass.
Authors: Lee, Young-Sam and Gregory, Mark T and Yang, Wei
Journal: Proceedings of the National Academy of Sciences of the United States of America (2014): 2954-9

[Detection of heterogeneity and evolution of subclones in t(8;21) AML by QM-FISH].
Authors: Wang, Ying-chan and Hu, Lin-ping and Lin, Dong and Li, Cheng-wen and Yuan, Tian and Jia, Yu-jiao and Tian, Zheng and Tang, Ke-jing and Wang, Min and Wang, Jian-xiang
Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (2013): 844-50

A comprehensive HBV array for the detection of HBV mutants and genotype.
Authors: Chan, Kaimin and Yam, Irene and Yuen, John and Yuen, Man-Fung and Lai, Ching-Lung and Alexander, Graeme J and Chan, Tai-Kwong and Chan, Vivian
Journal: Clinical biochemistry (2011): 1253-60

Cationic conjugated polymers for optical detection of DNA methylation, lesions, and single nucleotide polymorphisms.
Authors: Duan, Xinrui and Liu, Libing and Feng, Fude and Wang, Shu
Journal: Accounts of chemical research (2010): 260-70

Page updated on November 21, 2024

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Catalog Number	17035
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Physical properties

Molecular weight	991.69
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501