Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7.5)*
Ordering information
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Additional ordering information
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Physical properties
Molecular weight | 1014.72 |
Solvent | Water |
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Molecular weight 1014.72 | Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
Fluorescein-12-dATP might be incorporated into a DNA sequence as its natural counterpart dATP to incorporate a fluorescein tag in a DNA molecule. It can be used for the direct enzymatic labeling of DNA/cDNA, e.g., by nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. The resulting fluorescein-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. The linker between fluorescein tag and adenine has been optimized for the optimal substrate incorporation and labeling efficiency. The labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.549 µL | 492.747 µL | 985.494 µL | 4.927 mL | 9.855 mL |
5 mM | 19.71 µL | 98.549 µL | 197.099 µL | 985.494 µL | 1.971 mL |
10 mM | 9.855 µL | 49.275 µL | 98.549 µL | 492.747 µL | 985.494 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Ffluorescein.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Ffluorescein.png&w=2048&q=50)
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Fluorescein-12-dUTP *1 mM in TE Buffer (pH 7.5)* *CAS 214154-36-6* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Fluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
References
View all 1 references: Citation Explorer
Highly sensitive fluorometric determination of thrombin by on-chip signal amplification initiated by terminal deoxynucleotidyl transferase.
Authors: Wen, Dongxiao and He, Minhui and Ma, Kefeng and Cui, Ying and Kong, Jinming and Yang, Huaixia and Liu, Qingyun
Journal: Mikrochimica acta (2018): 380
Authors: Wen, Dongxiao and He, Minhui and Ma, Kefeng and Cui, Ying and Kong, Jinming and Yang, Huaixia and Liu, Qingyun
Journal: Mikrochimica acta (2018): 380
Application notes
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