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Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7.5)*
Fluorescein-12-dATP might be incorporated into a DNA sequence as its natural counterpart dATP to incorporate a fluorescein tag in a DNA molecule. It can be used for the direct enzymatic labeling of DNA/cDNA, e.g., by nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. The resulting fluorescein-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. The linker between fluorescein tag and adenine has been optimized for the optimal substrate incorporation and labeling efficiency. The labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Fluorescein-12-dUTP *1 mM in TE Buffer (pH 7.5)* *CAS 214154-36-6* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| Fluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
References
View all 1 references: Citation Explorer
Highly sensitive fluorometric determination of thrombin by on-chip signal amplification initiated by terminal deoxynucleotidyl transferase.
Authors: Wen, Dongxiao and He, Minhui and Ma, Kefeng and Cui, Ying and Kong, Jinming and Yang, Huaixia and Liu, Qingyun
Journal: Mikrochimica acta (2018): 380
Authors: Wen, Dongxiao and He, Minhui and Ma, Kefeng and Cui, Ying and Kong, Jinming and Yang, Huaixia and Liu, Qingyun
Journal: Mikrochimica acta (2018): 380
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