Fluorescein-12-dUTP 1 mM in Tris Buffer (pH 7.5)

The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-12-dUTP conjugate can be used as a green fluorescence color with Spectrum Green™ filter set (Spectrum Green™ is the trademark of Vysis).

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein-12-dCTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dATP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dGTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

References

View all 50 references: Citation Explorer

Effects of Aerobic, Resistance, and Combined Exercise on Markers of Male Reproduction in Healthy Human Subjects: A Randomized Controlled Trial.
Authors: Hajizadeh Maleki, Behzad and Tartibian, Bakhtyar and Chehrazi, Mohammad
Journal: Journal of strength and conditioning research (2019): 1130-1145

Fluorescently Labeled DNA Interacts with Competence and Recombination Proteins and Is Integrated and Expressed Following Natural Transformation of Bacillus subtilis.
Authors: Boonstra, Mirjam and Vesel, Nina and Kuipers, Oscar P
Journal: mBio (2018)

TUNEL labeling with BrdUTP/anti-BrdUTP greatly underestimates the level of sperm DNA fragmentation in semen evaluation.
Authors: Ribeiro, Sofia C and Muratori, Monica and De Geyter, Maria and De Geyter, Christian
Journal: PloS one (2017): e0181802

Role of carvacrol in cardioprotection against myocardial ischemia/reperfusion injury in rats through activation of MAPK/ERK and Akt/eNOS signaling pathways.
Authors: Chen, Yunping and Ba, Lina and Huang, Wei and Liu, Yan and Pan, Hao and Mingyao, E and Shi, Pilong and Wang, Ye and Li, Shuzhi and Qi, Hanping and Sun, Hongli and Cao, Yonggang
Journal: European journal of pharmacology (2017): 90-100

Role of insulin-like growth factor 1 on cross-bred Bos indicus cattle germinal vesicle oocytes exposed to heat shock.
Authors: Lima, Rafaela S and Risolia, Pedro H B and Ispada, Jéssica and Assumpção, Mayra E O A and Visintin, José A and Orlandi, Cássia and Paula-Lopes, Fabíola F
Journal: Reproduction, fertility, and development (2017): 1405-1414

Page updated on July 26, 2023

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Catalog Number	17028
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Physical properties

Molecular weight	1060.63
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501