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EthD-2 [Ethidium Homodimer-2]
Ethidium Homodimer-2 (EthD-2) is usually used as a cell-impermeant viability indicator. It is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~535/624). It is often used together with Calcein AM. The viability of cells can be readily assessed by staining with calcein acetoxymethyl ester (Calcein AM) and EthD-2. The live cells (green, stained with Calcein AM) and dead cells (red, stained with EthD-2) can be easily imaged and even quantified from the images using the ImageJ program. It might also be combined with our other cell viability stains such as the superior Calcein Ultra Blue, Calcein Ultra Green, Cytocalcein, Cytotrace dyes and CytoTell dyes. AAT Bioquest offers the largest collection of fluorescent stains for monitoring cell viabilities.
Product family
| Name | Excitation (nm) | Emission (nm) |
| 2-Aminoacridone *CAS 27918-14-5* | 429 | 529 |
| BTA-2 [2-(4'-(dimethylamino)phenyl)-6-methyl-benzothiazole] | 355 | 426 |
| 2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] *CAS#: 186689-07-6* | 467 | 538 |
| EthD-1 [Ethidium Homodimer-1] | 528 | 617 |
References
View all 15 references: Citation Explorer
Iatrogenic Articular Cartilage Injury in Arthroscopic Hip and Knee Videos and the Potential for Cartilage Cell Death When Simulated in a Bovine Model.
Authors: Compton, Jocelyn and Slattery, Michael and Coleman, Mitchell and Westermann, Robert
Journal: Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North Ameri (2020): 2114-2121
Authors: Compton, Jocelyn and Slattery, Michael and Coleman, Mitchell and Westermann, Robert
Journal: Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North Ameri (2020): 2114-2121
In Vitro Effect of Vancomycin on the Viability of Articular Chondrocytes.
Authors: Shaw, K Aaron and Eichinger, Josef K and Nadig, Nischal and Parada, Stephen A
Journal: Journal of orthopaedic trauma (2018): 148-153
Authors: Shaw, K Aaron and Eichinger, Josef K and Nadig, Nischal and Parada, Stephen A
Journal: Journal of orthopaedic trauma (2018): 148-153
Chondrocyte Viability After a Simulated Blast Exposure.
Authors: Shaw, K Aaron and Johnson, Peter C and Williams, David and Zumbrun, Steven D and Topolski, Richard and Cameron, Craig D
Journal: Military medicine (2017): e1941-e1947
Authors: Shaw, K Aaron and Johnson, Peter C and Williams, David and Zumbrun, Steven D and Topolski, Richard and Cameron, Craig D
Journal: Military medicine (2017): e1941-e1947
Why Do Osteochondral Allografts Survive? Comparative Analysis of Cartilage Biochemical Properties Unveils a Molecular Basis for Durability.
Authors: Ding, Lei and Zampogna, Biagio and Vasta, Sebastiano and Jang, Kee Woong and De Caro, Francesca and Martin, James A and Amendola, Annunziato
Journal: The American journal of sports medicine (2015): 2459-68
Authors: Ding, Lei and Zampogna, Biagio and Vasta, Sebastiano and Jang, Kee Woong and De Caro, Francesca and Martin, James A and Amendola, Annunziato
Journal: The American journal of sports medicine (2015): 2459-68
DNA counterstaining for methylation and hydroxymethylation immunostaining in bovine zygotes.
Authors: Heras, Sonia and Forier, Katrien and Rombouts, Koen and Braeckmans, Kevin and Van Soom, Ann
Journal: Analytical biochemistry (2014): 14-6
Authors: Heras, Sonia and Forier, Katrien and Rombouts, Koen and Braeckmans, Kevin and Van Soom, Ann
Journal: Analytical biochemistry (2014): 14-6
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