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2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] *CAS#: 186689-07-6*
2-NBDG is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] *CAS#: 186689-07-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 292.176 µL | 1.461 mL | 2.922 mL | 14.609 mL | 29.218 mL |
| 5 mM | 58.435 µL | 292.176 µL | 584.351 µL | 2.922 mL | 5.844 mL |
| 10 mM | 29.218 µL | 146.088 µL | 292.176 µL | 1.461 mL | 2.922 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| 2-Aminoacridone *CAS 27918-14-5* | 429 | 529 |
| BTA-2 [2-(4'-(dimethylamino)phenyl)-6-methyl-benzothiazole] | 355 | 426 |
Citations
View all 9 citations: Citation Explorer
FGF21 alleviates adipose stem cell senescence via CD90 glycosylation-dependent glucose influx in remodeling healthy white adipose tissue
Authors: Zhou, Zixin and Zhang, Huiying and Tao, Yan and Zang, Jinhao and Zhao, Jingyuan and Li, Huijie and Wang, Yalin and Wang, Tianci and Zhao, Hui and Wang, Fuwu and others,
Journal: Redox Biology (2023): 102877
Authors: Zhou, Zixin and Zhang, Huiying and Tao, Yan and Zang, Jinhao and Zhao, Jingyuan and Li, Huijie and Wang, Yalin and Wang, Tianci and Zhao, Hui and Wang, Fuwu and others,
Journal: Redox Biology (2023): 102877
GLP-1RA Liraglutide and Semaglutide Improves Obesity-Induced Muscle Atrophy via SIRT1 Pathway
Authors: Xiang, Jie and Qin, Liyan and Zhong, Jinling and Xia, Ning and Liang, Yuzhen
Journal: Diabetes, Metabolic Syndrome and Obesity (2023): 2433--2446
Authors: Xiang, Jie and Qin, Liyan and Zhong, Jinling and Xia, Ning and Liang, Yuzhen
Journal: Diabetes, Metabolic Syndrome and Obesity (2023): 2433--2446
Inhibition of human CD8+ T cells by the tumor metabolite methylthioadenosine: Mechanisms of action and putative strategies to improve antitumor activity
Authors: Strobl, Carolin Dorothea
Journal: (2022)
Authors: Strobl, Carolin Dorothea
Journal: (2022)
Metabolische und funktionelle Modulation von Effektor-T-Zellen durch humane doppelt-negative T-Zellen
Authors: Haug, Tabea
Journal: (2020)
Authors: Haug, Tabea
Journal: (2020)
Selective PRMT5 inhibitors suppress human CD8+ T cells by upregulation of p53 and impairment of the AKT pathway similar to the tumor metabolite MTA
Authors: Strobl, Carolin Dorothea and Schaffer, Stefanie and Haug, Tabea and V{\"o}lkl, Simon and Peter, Katrin and Singer, Katrin and B{\"o}ttcher, Martin and Mougiakakos, Dimitrios and Mackensen, Andreas and Aigner, Michael
Journal: Molecular cancer therapeutics (2020): 409--419
Authors: Strobl, Carolin Dorothea and Schaffer, Stefanie and Haug, Tabea and V{\"o}lkl, Simon and Peter, Katrin and Singer, Katrin and B{\"o}ttcher, Martin and Mougiakakos, Dimitrios and Mackensen, Andreas and Aigner, Michael
Journal: Molecular cancer therapeutics (2020): 409--419
References
View all 14 references: Citation Explorer
Transport of a Fluorescent Analogue of Glucose (2-NBDG) versus Radiolabeled Sugars by Rumen Bacteria and Escherichia coli
Authors: Tao J, Diaz RK, Teixeira CR, Hackmann TJ.
Journal: Biochemistry (2016): 2578
Authors: Tao J, Diaz RK, Teixeira CR, Hackmann TJ.
Journal: Biochemistry (2016): 2578
2-NBDG as a marker for detecting glucose uptake in reactive astrocytes exposed to oxygen-glucose deprivation in vitro
Authors: Chen Y, Zhang J, Zhang XY.
Journal: J Mol Neurosci (2015): 126
Authors: Chen Y, Zhang J, Zhang XY.
Journal: J Mol Neurosci (2015): 126
2-NBDG fluorescence imaging of hypermetabolic circulating tumor cells in mouse xenograft model of breast cancer
Authors: Cai H, Peng F.
Journal: J Fluoresc (2013): 213
Authors: Cai H, Peng F.
Journal: J Fluoresc (2013): 213
Syzygium aqueum leaf extract and its bioactive compounds enhances pre-adipocyte differentiation and 2-NBDG uptake in 3T3-L1 cells
Authors: Manaharan T, Ming CH, Palanisamy UD.
Journal: Food Chem (2013): 354
Authors: Manaharan T, Ming CH, Palanisamy UD.
Journal: Food Chem (2013): 354
In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog
Authors: Tsytsarev V, Maslov KI, Yao J, Parameswar AR, Demchenko AV, Wang LV.
Journal: J Neurosci Methods (2012): 136
Authors: Tsytsarev V, Maslov KI, Yao J, Parameswar AR, Demchenko AV, Wang LV.
Journal: J Neurosci Methods (2012): 136
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