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EthD-1 [Ethidium Homodimer-1]
Ethidium Homodimer-1 (EthD-1) is a high-affinity fluorescent nucleic acid dye that binds ssDNA, dsDNA, RNA, oligonucleotides and triple helix DNA. Upon binding DNAs, its fluorescence is significantly enhanced (>30 times), and bright red fluorescence is emitted. Since EthD-1 carries a strong positive charge and cannot penetrate intact/living cell membranes, it is often used to detect dead cells. EthD-1 is generally used in combination with Calcein AM to stain dead and live cells respectively to determine cell viability and toxicity. EthD-1 is a dead cell-specific dye in all cell types (including mammals, bacteria and yeast). It might also be used for monitoring live/dead cell tests in combination with our other cell viability stains such as the superior Calcein Ultra Blue, Calcein Ultra Green, Cytocalcein, Cytotrace dyes and CytoTell dyes. AAT Bioquest offers the largest collection of fluorescent stains for monitoring cell viabilities.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 |
| DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 |
| DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 |
| JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 |
| BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - |
| DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 |
| DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 |
References
View all 50 references: Citation Explorer
Designing of spider silk proteins for human induced pluripotent stem cell-based cardiac tissue engineering.
Authors: Esser, T U and Trossmann, V T and Lentz, S and Engel, F B and Scheibel, T
Journal: Materials today. Bio (2021): 100114
Authors: Esser, T U and Trossmann, V T and Lentz, S and Engel, F B and Scheibel, T
Journal: Materials today. Bio (2021): 100114
Designing and validation of an automated ex-vivo bioreactor system for long term culture of bone.
Authors: Dua, Rupak and Jones, Hugh and Noble, Philip C
Journal: Bone reports (2021): 101074
Authors: Dua, Rupak and Jones, Hugh and Noble, Philip C
Journal: Bone reports (2021): 101074
The Silk Protein Sericin Promotes Viability of ARPE-19 and Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells in vitro.
Authors: Khan, Ayyad Zartasht and Utheim, Tor Paaske and Moe, Morten Carstens and Aass, Hans Christian D and Sapkota, Dipak and Vallenari, Evan Michael and Eidet, Jon Roger
Journal: Current eye research (2021): 504-514
Authors: Khan, Ayyad Zartasht and Utheim, Tor Paaske and Moe, Morten Carstens and Aass, Hans Christian D and Sapkota, Dipak and Vallenari, Evan Michael and Eidet, Jon Roger
Journal: Current eye research (2021): 504-514
Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model.
Authors: Mamani, Javier Bustamante and Marinho, Bruna Souto and Rego, Gabriel Nery de Albuquerque and Nucci, Mariana Penteado and Alvieri, Fernando and Santos, Ricardo Silva Dos and Ferreira, João Victor Matias and Oliveira, Fernando Anselmo de and Gamarra, Lionel Fernel
Journal: Einstein (Sao Paulo, Brazil) (2020): eAO4954
Authors: Mamani, Javier Bustamante and Marinho, Bruna Souto and Rego, Gabriel Nery de Albuquerque and Nucci, Mariana Penteado and Alvieri, Fernando and Santos, Ricardo Silva Dos and Ferreira, João Victor Matias and Oliveira, Fernando Anselmo de and Gamarra, Lionel Fernel
Journal: Einstein (Sao Paulo, Brazil) (2020): eAO4954
Influence of Tooth Pigmentation on H2O2 Diffusion and Its Cytotoxicity After In-office Tooth Bleaching.
Authors: de Oliveira Duque, C C and Soares, D G and Briso, Alf and Ortecho-Zuta, U and de Oliveira Ribeiro, R A and Hebling, J and de Souza Costa, C A
Journal: Operative dentistry (2020): 632-642
Authors: de Oliveira Duque, C C and Soares, D G and Briso, Alf and Ortecho-Zuta, U and de Oliveira Ribeiro, R A and Hebling, J and de Souza Costa, C A
Journal: Operative dentistry (2020): 632-642
Page updated on July 26, 2023
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