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DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide]
DiI, DiO, DiS, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 182.973 µL | 914.863 µL | 1.83 mL | 9.149 mL | 18.297 mL |
| 5 mM | 36.595 µL | 182.973 µL | 365.945 µL | 1.83 mL | 3.659 mL |
| 10 mM | 18.297 µL | 91.486 µL | 182.973 µL | 914.863 µL | 1.83 mL |
Molarity calculator
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Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.32 | 0.178 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.32 | 0.178 |
| 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | - | 0.35 |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.32 | 0.35 |
| 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* | 498 | 517 | 800001 | 0.32 | 0.35 |
| 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.24 | 0.091 |
| 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.24 | 0.214 |
| 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.32 | 0.178 |
| 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | - | 0.168 |
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Citations
View all 55 citations: Citation Explorer
Bactericidal and anti-quorum sensing activity of repurposing drug visomitin against staphylococcus aureus
Authors: Wu, Ruolan and Wu, Yuan and Wu, Pingyun and Li, Huilong and She, Pengfei
Journal: Virulence (2024): 2415952
Authors: Wu, Ruolan and Wu, Yuan and Wu, Pingyun and Li, Huilong and She, Pengfei
Journal: Virulence (2024): 2415952
Synergistic collaboration between AMPs and non-direct antimicrobial cationic peptides
Authors: Ye, Zifan and Fu, Lei and Li, Shuangyu and Chen, Ziying and Ouyang, Jianhong and Shang, Xinci and Liu, Yanli and Gao, Lianghui and Wang, Yipeng
Journal: Nature Communications (2024): 7319
Authors: Ye, Zifan and Fu, Lei and Li, Shuangyu and Chen, Ziying and Ouyang, Jianhong and Shang, Xinci and Liu, Yanli and Gao, Lianghui and Wang, Yipeng
Journal: Nature Communications (2024): 7319
Rapid Membrane-Penetrating Hybrid Peptides Achieve Efficient Dual Antimicrobial and Antibiofilm Activity through a Triple Bactericidal Mechanism
Authors: Liu, Yifan and Cui, Pengfei and Tan, Rong and Ru, Shaoguo
Journal: ACS Omega (2024)
Authors: Liu, Yifan and Cui, Pengfei and Tan, Rong and Ru, Shaoguo
Journal: ACS Omega (2024)
Flagellar pH homeostasis mediated by Na+/H+ exchangers regulates human sperm functions through coupling with CatSper and KSper activation
Authors: Liang, Min and Ji, Nanxi and Song, Jian and Kang, Hang and Zeng, Xuhui
Journal: Human Reproduction (2024): deae020
Authors: Liang, Min and Ji, Nanxi and Song, Jian and Kang, Hang and Zeng, Xuhui
Journal: Human Reproduction (2024): deae020
The enhancement effect of small molecule Lyb24 reveals AzoR as a novel target of polymyxin B
Authors: Hu, Chunxia and Zhang, Jinyong and Cui, Ruiqin and Liu, Shiyi and Huang, Ying and Zeng, Huan and Cheng, Shumin and Zhou, Guibao and Li, Jingli and Sun, Longqin and others,
Journal: Biomedicine \& Pharmacotherapy (2023): 115856
Authors: Hu, Chunxia and Zhang, Jinyong and Cui, Ruiqin and Liu, Shiyi and Huang, Ying and Zeng, Huan and Cheng, Shumin and Zhou, Guibao and Li, Jingli and Sun, Longqin and others,
Journal: Biomedicine \& Pharmacotherapy (2023): 115856
References
View all 92 references: Citation Explorer
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
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Journal: Hypertension (2006): 482
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
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