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DiSBAC2(5) [Bis-(1,3-diethylthiobarbituric acid)pentamethine oxonol]
DiSBAC2(5) is a sensitive slow-response membrane potential probe that is widely used for measuring membrane potentials of many biological systems. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiSBAC2(5) [Bis-(1,3-diethylthiobarbituric acid)pentamethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 216.174 µL | 1.081 mL | 2.162 mL | 10.809 mL | 21.617 mL |
| 5 mM | 43.235 µL | 216.174 µL | 432.348 µL | 2.162 mL | 4.323 mL |
| 10 mM | 21.617 µL | 108.087 µL | 216.174 µL | 1.081 mL | 2.162 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
| 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
| 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* | 498 | 517 | 800001 | 0.35 |
| 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
| 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
| 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
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Citations
View all 1 citations: Citation Explorer
Suppression of K V 7/KCNQ potassium channel enhances neuronal differentiation of PC12 cells
Authors: Zhou, Najing and Huang, Sha and Li, Li and Huang, Dongyang and Yan, Yunli and Du, Xiaona and Zhang, Hailin
Journal: Neuroscience (2016): 356--367
Authors: Zhou, Najing and Huang, Sha and Li, Li and Huang, Dongyang and Yan, Yunli and Du, Xiaona and Zhang, Hailin
Journal: Neuroscience (2016): 356--367
References
View all 3 references: Citation Explorer
Comparative study of membrane potential-sensitive fluorescent probes and their use in ion channel screening assays
Authors: Wolff C, Fuks B, Chatelain P.
Journal: J Biomol Screen (2003): 533
Authors: Wolff C, Fuks B, Chatelain P.
Journal: J Biomol Screen (2003): 533
Characterization of the taurine transport pathway in A6 kidney cells
Authors: Schmieder S, Soriani O, Brochiero E, Raschi C, Bogliolo S, Lindenthal S, Ehrenfeld J.
Journal: J Membr Biol (2002): 145
Authors: Schmieder S, Soriani O, Brochiero E, Raschi C, Bogliolo S, Lindenthal S, Ehrenfeld J.
Journal: J Membr Biol (2002): 145
Membrane potential changes after infection of monocytes by Toxoplasma gondii
Authors: Bouchot A, Millot JM, Charpentier S, Bonhomme A, Villena I, Aubert D, Pinon JM.
Journal: Int J Parasitol (2001): 1114
Authors: Bouchot A, Millot JM, Charpentier S, Bonhomme A, Villena I, Aubert D, Pinon JM.
Journal: Int J Parasitol (2001): 1114
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