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DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol]
DiBAC4(5) is a sensitive membrane potential probe with longer excitation and emission wavelength. It is a slow-response probe for measuring cellular membrane potential. In general, slow-response probes exhibit potential-dependent changes in their transmembrane distribution that are accompanied by a fluorescence change. The magnitude of their optical responses is much larger than that of fast-response probes (typically a 1% fluorescence change per mV). Slow-response probes, which include cationic carbocyanines, rhodamines and anionic oxonols, are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiBAC4(5) [Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 184.274 µL | 921.37 µL | 1.843 mL | 9.214 mL | 18.427 mL |
| 5 mM | 36.855 µL | 184.274 µL | 368.548 µL | 1.843 mL | 3.685 mL |
| 10 mM | 18.427 µL | 92.137 µL | 184.274 µL | 921.37 µL | 1.843 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.178 |
| 5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.178 |
| 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | 0.35 |
| 5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.35 |
| 5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* | 498 | 517 | 800001 | 0.35 |
| 5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.091 |
| 5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.214 |
| 5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.178 |
| 5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | 0.168 |
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Citations
View all 5 citations: Citation Explorer
A method for quantifying sporulation efficiency and isolating meiotic progeny in non-GMO strains of Saccharomyces cerevisiae
Authors: Sirr, Amy and Timour, Martin S and Cromie, Gareth A and Tang, Michelle and Dudley, Aim{\'e}e M
Journal: Yeast (2022): 354--362
Authors: Sirr, Amy and Timour, Martin S and Cromie, Gareth A and Tang, Michelle and Dudley, Aim{\'e}e M
Journal: Yeast (2022): 354--362
A Membrane Potential-and Calpain-Dependent Reversal of Caspase-1 Inhibition Regulates Canonical NLRP3 Inflammasome
Authors: Zhang, Yifei and Rong, Hua and Zhang, Fang-Xiong and Wu, Kun and Mu, Libing and Meng, Junchen and Xiao, Bailong and Zamponi, Gerald W and Shi, Yan
Journal: Cell reports (2018): 2356--2369
Authors: Zhang, Yifei and Rong, Hua and Zhang, Fang-Xiong and Wu, Kun and Mu, Libing and Meng, Junchen and Xiao, Bailong and Zamponi, Gerald W and Shi, Yan
Journal: Cell reports (2018): 2356--2369
Effects of 1, 4-naphthoquinone Aged Carbon Black Particles on the Cell Membrane of Human Bronchial Epithelium
Authors: Zhang, Yongming and Wang, Li and Feng, Huimin and Hu, Guiping and Wang, Lele and Liu, Jiaxing and Gao, Xin and Shang, Jing and Zhu, Tong and Tang, Shichuan and others, undefined
Journal: Environmental Toxicology and Pharmacology (2017)
Authors: Zhang, Yongming and Wang, Li and Feng, Huimin and Hu, Guiping and Wang, Lele and Liu, Jiaxing and Gao, Xin and Shang, Jing and Zhu, Tong and Tang, Shichuan and others, undefined
Journal: Environmental Toxicology and Pharmacology (2017)
Humic analog AQDS can act as a selective inhibitor to enable anoxygenic photosynthetic bacteria to outcompete sulfate-reducing bacteria under microaerobic conditions
Authors: Wang, Xingzu and Cheng, Xiang and Ren, Yiwei and Xu, Guihua and Tang, Jing
Journal: Journal of Chemical Technology and Biotechnology (2015)
Authors: Wang, Xingzu and Cheng, Xiang and Ren, Yiwei and Xu, Guihua and Tang, Jing
Journal: Journal of Chemical Technology and Biotechnology (2015)
Mitochondrial ROS-K+ channel signaling pathway regulated secretion of human pulmonary artery endothelial cells
Authors: Ouyang, Jin-Sheng and Li, Yu-Ping and Li, Cheng-Ye and Cai, Chang and Chen, Cheng-Shui and Chen, Shao-Xian and Chen, Yan-Fan and Yang, Li and Xie, Yu-Peng
Journal: Free radical research (2012): 1437--1445
Authors: Ouyang, Jin-Sheng and Li, Yu-Ping and Li, Cheng-Ye and Cai, Chang and Chen, Cheng-Shui and Chen, Shao-Xian and Chen, Yan-Fan and Yang, Li and Xie, Yu-Peng
Journal: Free radical research (2012): 1437--1445
References
View all 36 references: Citation Explorer
Role of cGMP in sildenafil-induced activation of endothelial Ca2+-activated K+ channels
Authors: Luedders DW, Muenz BM, Li F, Rueckleben S, Tillmanns H, Waldecker B, Wiecha J, Erdogan A, Schaefer CA, Kuhlmann CR.
Journal: J Cardiovasc Pharmacol (2006): 365
Authors: Luedders DW, Muenz BM, Li F, Rueckleben S, Tillmanns H, Waldecker B, Wiecha J, Erdogan A, Schaefer CA, Kuhlmann CR.
Journal: J Cardiovasc Pharmacol (2006): 365
Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes
Authors: Papadimitriou K, Pratsinis H, Nebe-von-Caron G, Kletsas D, Tsakalidou E.
Journal: Int J Food Microbiol (2006): 197
Authors: Papadimitriou K, Pratsinis H, Nebe-von-Caron G, Kletsas D, Tsakalidou E.
Journal: Int J Food Microbiol (2006): 197
N-terminal parathyroid hormone-related peptide hyperpolarizes endothelial cells and causes a reduction of the coronary resistance of the rat heart via endothelial hyperpolarization
Authors: Abdallah Y, Ross G, Dolf A, Heinemann MP, Schluter KD.
Journal: Peptides (2006): 2927
Authors: Abdallah Y, Ross G, Dolf A, Heinemann MP, Schluter KD.
Journal: Peptides (2006): 2927
The Zn2+-transporting pathways in pancreatic beta-cells: a role for the L-type voltage-gated Ca2+ channel
Authors: Gyulkh, undefined and anyan AV, Lee SC, Bikopoulos G, Dai F, Wheeler MB.
Journal: J Biol Chem (2006): 9361
Authors: Gyulkh, undefined and anyan AV, Lee SC, Bikopoulos G, Dai F, Wheeler MB.
Journal: J Biol Chem (2006): 9361
Activation of Ca(2+)-activated potassium channels is involved in lysophosphatidylcholine-induced monocyte adhesion to endothelial cells
Authors: Erdogan A, Schaefer MB, Kuhlmann CR, Most A, Hartmann M, Mayer K, Renner FC, Schaefer C, Abdallah Y, Hoelschermann H, Schaefer CA.
Journal: Atherosclerosis. (2006)
Authors: Erdogan A, Schaefer MB, Kuhlmann CR, Most A, Hartmann M, Mayer K, Renner FC, Schaefer C, Abdallah Y, Hoelschermann H, Schaefer CA.
Journal: Atherosclerosis. (2006)
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