Cal-520®, potassium salt
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Dissociation constant (Kd, nM) | 320 |
Molecular weight | 921.08 |
Solvent | Water |
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Calcium Indicators, Multi-Photon Microscopy
Molecular weight 921.08 | Dissociation constant (Kd, nM) 320 | Excitation (nm) 492 | Emission (nm) 515 | Quantum yield 0.751 |
Cal-520® provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520® AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520® AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Cal-520™ AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520®. The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520® AM an ideal indicator for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520® calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists. Cal-520® sodium or potassium salt is the hydrolyzed salt of Cal-520® AM in cells. It selectively binds to calcium ion, and has the fluorescence that is strongly dependent on calcium concentration.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cal-520®, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 108.568 µL | 542.841 µL | 1.086 mL | 5.428 mL | 10.857 mL |
5 mM | 21.714 µL | 108.568 µL | 217.136 µL | 1.086 mL | 2.171 mL |
10 mM | 10.857 µL | 54.284 µL | 108.568 µL | 542.841 µL | 1.086 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcal_520.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcal_520.png&w=2048&q=50)
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Product Family
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Cal-520®, sodium salt | 492 | 515 | 0.751 |
Cal-520FF™, potassium salt | 492 | 515 | 0.751 |
Cal-520N™, potassium salt | 492 | 515 | 0.751 |
Cal-590™, potassium salt | 574 | 588 | 0.621 |
Cal-630™, potassium salt | 609 | 626 | 0.371 |
Calbryte™ 520, potassium salt | 493 | 515 | 0.751 |
Cal-500™, potassium salt | 388 | 482 | 0.481 |
Cal-670™, potassium salt | 667 | 680 | - |
Cal-770™, potassium salt | 758 | 783 | - |
Show More (1) |
Images
![ATP-stimulated calcium responses of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-520™ AM (red curve), or Fluo-4 AM (blue curve) respectively with (left) or without probenecid (right) under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 µL per well in a Costar black wall/clear bottom 96-well plate. 100 µL of 5 µM Fluo-4 AM or Cal 520™ AM in HHBS (with or without 2.5 mm probenecid) was added into the cells, and the cells were incubated at 37 °C for 1 hour. ATP (50 μL/well) was added using FlexSation to achieve the final indicated concentrations.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fcal-520-potassium-salt%2Ffigure-for-cal-520-potassium-salt_sPLrp.jpg&w=3840&q=75)
Figure 1. ATP-stimulated calcium responses of endogenous P2Y receptor in CHO-K1 cells incubated with Cal-520™ AM (red curve), or Fluo-4 AM (blue curve) respectively with (left) or without probenecid (right) under the same conditions. CHO-K1 cells were seeded overnight at 50,000 cells per 100 µL per well in a Costar black wall/clear bottom 96-well plate. 100 µL of 5 µM Fluo-4 AM or Cal 520™ AM in HHBS (with or without 2.5 mm probenecid) was added into the cells, and the cells were incubated at 37 °C for 1 hour. ATP (50 μL/well) was added using FlexSation to achieve the final indicated concentrations.
Citations
View all 101 citations: Citation Explorer
Enhanced long-term potentiation in the anterior cingulate cortex of tree shrew
Authors: Song, Qian and Li, Xu-Hui and Lu, Jing-Shan and Chen, Qi-Yu and Liu, Ren-Hao and Zhou, Si-Bo and Zhuo, Min
Journal: Philosophical Transactions B (2024): 20230240
Authors: Song, Qian and Li, Xu-Hui and Lu, Jing-Shan and Chen, Qi-Yu and Liu, Ren-Hao and Zhou, Si-Bo and Zhuo, Min
Journal: Philosophical Transactions B (2024): 20230240
Activation of the glutamatergic cingulate cortical-cortical connection facilitates pain in adult mice
Authors: Li, Xu-Hui and Shi, Wantong and Chen, Qi-Yu and Hao, Shun and Miao, Hui-Hui and Miao, Zhuang and Xu, Fang and Bi, Guo-Qiang and Zhuo, Min
Journal: Communications Biology (2023): 1247
Authors: Li, Xu-Hui and Shi, Wantong and Chen, Qi-Yu and Hao, Shun and Miao, Hui-Hui and Miao, Zhuang and Xu, Fang and Bi, Guo-Qiang and Zhuo, Min
Journal: Communications Biology (2023): 1247
L-type Ca2+ channel recovery from inactivation in rabbit atrial myocytes
Authors: Martinez-Hernandez, Elizabeth and Blatter, Lothar A and Kanaporis, Giedrius
Journal: Physiological Reports (2022): e15222
Authors: Martinez-Hernandez, Elizabeth and Blatter, Lothar A and Kanaporis, Giedrius
Journal: Physiological Reports (2022): e15222
Dendritic spikes in apical oblique dendrites of cortical layer 5 pyramidal neurons
Authors: Casta{\~n}ares, Michael Lawrence G and Stuart, Greg J and Daria, Vincent R
Journal: bioRxiv (2020)
Authors: Casta{\~n}ares, Michael Lawrence G and Stuart, Greg J and Daria, Vincent R
Journal: bioRxiv (2020)
Multiphoton minimal inertia scanning for fast acquisition of neural activity signals
Authors: Schuck, Renaud and Go, Mary Ann and Garasto, Stefania and Reynolds, Stephanie and Dragotti, Pier Luigi and Schultz, Simon R
Journal: Journal of neural engineering (2018): 025003
Authors: Schuck, Renaud and Go, Mary Ann and Garasto, Stefania and Reynolds, Stephanie and Dragotti, Pier Luigi and Schultz, Simon R
Journal: Journal of neural engineering (2018): 025003
Spatio-temporal modulation of light for stimulation and recording of neuronal activity
Authors: Ma, He and Castanares, Michael Lawrence and Daria, Vincent
Journal: (2018): 1072306
Authors: Ma, He and Castanares, Michael Lawrence and Daria, Vincent
Journal: (2018): 1072306
Characterization of postsynaptic calcium signals in the pyramidal neurons of anterior cingulate cortex
Authors: Li, Xu-Hui and Song, Qian and Chen, Tao and Zhuo, Min
Journal: Molecular pain (2017): 1744806917719847
Authors: Li, Xu-Hui and Song, Qian and Chen, Tao and Zhuo, Min
Journal: Molecular pain (2017): 1744806917719847
Two-photon minimal inertia scanning patterns for fast acquisition of calcium dynamics
Authors: Schuck, Renaud
Journal: (2017)
Authors: Schuck, Renaud
Journal: (2017)
Calcium Imaging Across Large Areas of Intact Vascular Endothelium Reveals Stimulus-Specific Sensory Cells
Authors: Lee, Matthew D and Wilson, Calum and McCarron, John G
Journal: The FASEB Journal (2017): 1005--8
Authors: Lee, Matthew D and Wilson, Calum and McCarron, John G
Journal: The FASEB Journal (2017): 1005--8
Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools
Authors: Daily, Neil J and Santos, Radleigh and Vecchi, Joseph and Kemanli, Pinar and Wakatsuki, Tetsuro
Journal: Journal of evolving stem cell research (2017): 1
Authors: Daily, Neil J and Santos, Radleigh and Vecchi, Joseph and Kemanli, Pinar and Wakatsuki, Tetsuro
Journal: Journal of evolving stem cell research (2017): 1
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?