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Cal-590™, potassium salt
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3® filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor® 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor® 488 labeled antibodies.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cal-590™, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 89.045 µL | 445.224 µL | 890.448 µL | 4.452 mL | 8.904 mL |
| 5 mM | 17.809 µL | 89.045 µL | 178.09 µL | 890.448 µL | 1.781 mL |
| 10 mM | 8.904 µL | 44.522 µL | 89.045 µL | 445.224 µL | 890.448 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Quantum yield |
| Cal-590™, sodium salt | 574 | 588 | 0.621 |
| Cal-630™, potassium salt | 609 | 626 | 0.371 |
| Cal-520®, potassium salt | 492 | 515 | 0.751 |
| Cal-520FF™, potassium salt | 492 | 515 | 0.751 |
| Cal-520N™, potassium salt | 492 | 515 | 0.751 |
| Calbryte™ 590, potassium salt | 581 | 593 | - |
| Cal-500™, potassium salt | 388 | 482 | 0.481 |
| Cal-670™, potassium salt | 667 | 680 | - |
| Cal-770™, potassium salt | 758 | 783 | - |
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Citations
View all 8 citations: Citation Explorer
Development of a deep two-photon calcium imaging method for the analysis of cortical processing in the mammalian brain
Authors: Birkner, Antje
Journal: (2019)
Authors: Birkner, Antje
Journal: (2019)
A versatile and Open-Source rapid LED switching system for One-Photon imaging and Photo-Activation
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2019): 530
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2019): 530
Gap junction coupling shapes the encoding of light in the developing retina
Authors: Caval-Holme, Franklin and Zhang, Yizhen and Feller, Marla B
Journal: Current Biology (2019): 4024--4035
Authors: Caval-Holme, Franklin and Zhang, Yizhen and Feller, Marla B
Journal: Current Biology (2019): 4024--4035
A versatile and open-source rapid LED switching system for one-photon imaging and photo-activation
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2018)
Authors: Battefeld, Arne and Popovic, Marko A and Van der Werf, Dirk and Kole, Maarten HP
Journal: Frontiers in cellular neuroscience (2018)
α V β 3 Integrin regulates astrocyte reactivity
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
Authors: Lagos-Cabré, Raúl and Alvarez, Alvaro and Kong, Milene and Burgos-Bravo, Francesca and Cárdenas, Areli and Rojas-Mancilla, Edgardo and Pérez-Nunez, Ramón and Herrera-Molina, Rodrigo and Rojas, Fabiola and Schneider, Pascal and others, undefined
Journal: Journal of Neuroinflammation (2017): 194
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