Cal-520® amine
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 909.68 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
Cal-520® maleimide |
Cal-520® azide |
Cal-520® alkyne |
Overview | SDSProtocol |
See also: Calcium Indicators
Molecular weight 909.68 | Excitation (nm) 492 | Emission (nm) 515 | Quantum yield 0.751 |
Cal-520® amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to a carboxy group via activated esters or EDC coupling chemistry. Calcium measurement is critical for numerous biological and neurological investigations. Fluorescent probes showing spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Cal-520® has been proven to be one of the best-known green fluorescent calcium indicators.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 109.929 µL | 549.644 µL | 1.099 mL | 5.496 mL | 10.993 mL |
5 mM | 21.986 µL | 109.929 µL | 219.858 µL | 1.099 mL | 2.199 mL |
10 mM | 10.993 µL | 54.964 µL | 109.929 µL | 549.644 µL | 1.099 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Images
References
View all 50 references: Citation Explorer
Oligosaccharide Blocks PAR1 (Proteinase-Activated Receptor 1)-PAR4-Mediated Platelet Activation by Binding to Thrombin Exosite II and Impairs Thrombosis.
Authors: Li, Sujuan and Wang, Weili and Lin, Lisha and Yang, Lian and Cai, Ying and Yang, Xingzhi and Zhang, Taocui and Xiao, Chuang and Yan, Hui and Gao, Na and Zhao, Jinhua
Journal: Arteriosclerosis, thrombosis, and vascular biology (2023): 253-266
Authors: Li, Sujuan and Wang, Weili and Lin, Lisha and Yang, Lian and Cai, Ying and Yang, Xingzhi and Zhang, Taocui and Xiao, Chuang and Yan, Hui and Gao, Na and Zhao, Jinhua
Journal: Arteriosclerosis, thrombosis, and vascular biology (2023): 253-266
The Calcium Transient Coupled to the L-Type Calcium Current Attenuates Action Potential Alternans.
Authors: Warren, Mark and Poelzing, Steven
Journal: bioRxiv : the preprint server for biology (2023)
Authors: Warren, Mark and Poelzing, Steven
Journal: bioRxiv : the preprint server for biology (2023)
Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer.
Authors: Romshin, Alexey M and Zeeb, Vadim and Glushkov, Evgenii and Radenovic, Aleksandra and Sinogeikin, Andrey G and Vlasov, Igor I
Journal: Scientific reports (2023): 8546
Authors: Romshin, Alexey M and Zeeb, Vadim and Glushkov, Evgenii and Radenovic, Aleksandra and Sinogeikin, Andrey G and Vlasov, Igor I
Journal: Scientific reports (2023): 8546
Bipartite Activation of Sensory Neurons by a TRPA1 Agonist Allyl Isothiocyanate Is Reflected by Complex Ca2+ Influx and CGRP Release Patterns: Enhancement by NGF and Inhibition with VAMP and SNAP-25 Cleaving Botulinum Neurotoxins.
Authors: Belinskaia, Mariia and Wang, Jiafu and Kaza, Seshu Kumar and Antoniazzi, Caren and Zurawski, Tomas and Dolly, J Oliver and Lawrence, Gary W
Journal: International journal of molecular sciences (2023)
Authors: Belinskaia, Mariia and Wang, Jiafu and Kaza, Seshu Kumar and Antoniazzi, Caren and Zurawski, Tomas and Dolly, J Oliver and Lawrence, Gary W
Journal: International journal of molecular sciences (2023)
FM1-43 Dye Memorizes Piezo1 Activation in the Trigeminal Nociceptive System Implicated in Migraine Pain.
Authors: Della Pietra, Adriana and Mikhailov, Nikita and Giniatullin, Rashid
Journal: International journal of molecular sciences (2023)
Authors: Della Pietra, Adriana and Mikhailov, Nikita and Giniatullin, Rashid
Journal: International journal of molecular sciences (2023)
BIN1, Myotubularin, and Dynamin-2 Coordinate T-Tubule Growth in Cardiomyocytes.
Authors: Perdreau-Dahl, Harmonie and Lipsett, David B and Frisk, Michael and Kermani, Fatemeh and Carlson, Cathrine R and Brech, Andreas and Shen, Xin and Bergan-Dahl, Anna and Hou, Yufeng and Tuomainen, Tomi and Tavi, Pasi and Jones, Peter P and Lunde, Marianne and Wasserstrom, J Andrew and Laporte, Jocelyn and Ullrich, Nina D and Christensen, Geir and Morth, J Preben and Louch, William E
Journal: Circulation research (2023): e188-e205
Authors: Perdreau-Dahl, Harmonie and Lipsett, David B and Frisk, Michael and Kermani, Fatemeh and Carlson, Cathrine R and Brech, Andreas and Shen, Xin and Bergan-Dahl, Anna and Hou, Yufeng and Tuomainen, Tomi and Tavi, Pasi and Jones, Peter P and Lunde, Marianne and Wasserstrom, J Andrew and Laporte, Jocelyn and Ullrich, Nina D and Christensen, Geir and Morth, J Preben and Louch, William E
Journal: Circulation research (2023): e188-e205
Capsazepine antagonizes TRPV1 activation induced by thermal and osmotic stimuli in human odontoblast-like cells.
Authors: Bernal-Cepeda, Lilia Jadith and Velandia-Romero, Myriam L and Castellanos, Jaime E
Journal: Journal of oral biology and craniofacial research (2023): 71-77
Authors: Bernal-Cepeda, Lilia Jadith and Velandia-Romero, Myriam L and Castellanos, Jaime E
Journal: Journal of oral biology and craniofacial research (2023): 71-77
Vascular Smooth Muscle TRPV4 (Transient Receptor Potential Vanilloid Family Member 4) Channels Regulate Vasoconstriction and Blood Pressure in Obesity.
Authors: Zhu, Yifei and Chu, Yuan and Wang, Sheng and Tang, Junjian and Li, Hu and Feng, Lei and Yu, Fan and Ma, Xin
Journal: Hypertension (Dallas, Tex. : 1979) (2023): 757-770
Authors: Zhu, Yifei and Chu, Yuan and Wang, Sheng and Tang, Junjian and Li, Hu and Feng, Lei and Yu, Fan and Ma, Xin
Journal: Hypertension (Dallas, Tex. : 1979) (2023): 757-770
Effects of apocynin on ISO-induced delayed afterdepolarizations in rat atrial myocytes and the underlying mechanisms.
Authors: Zhang, Nan and Dan, Qing and Dong, Ying and Liu, Yan and Zhuang, Wenjuan and Xie, Zhonghui and Zhao, Qianqian and Lin, Kun and Li, Yang
Journal: Biochemical and biophysical research communications (2023): 36-42
Authors: Zhang, Nan and Dan, Qing and Dong, Ying and Liu, Yan and Zhuang, Wenjuan and Xie, Zhonghui and Zhao, Qianqian and Lin, Kun and Li, Yang
Journal: Biochemical and biophysical research communications (2023): 36-42
The Mechanism of the Anti-Cardiac Hypertrophy Effect of Glycyrrhizic Acid Is Related to Reducing STIM1-Dependent Store-Operated Calcium Entry.
Authors: Cheng, X and Huang, L
Journal: Bulletin of experimental biology and medicine (2023): 701-706
Authors: Cheng, X and Huang, L
Journal: Bulletin of experimental biology and medicine (2023): 701-706
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any substitutes for probenecid in calcium assays?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Are there any substitutes for probenecid in calcium assays?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?