Cal-520® maleimide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 946.77 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Alternative formats
Cal-520® amine |
Cal-520® azide |
Cal-520® alkyne |
Overview | ![]() ![]() |
See also: Calcium Indicators, Multi-Photon Microscopy
Molecular weight 946.77 | Excitation (nm) 492 | Emission (nm) 515 | Quantum yield 0.751 |
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cal-520 has been proven to be the best green fluorescent calcium indicator. Conceivably this thiol-reactive Cal-520 maleimide could be coupled to an IgG or other carrier molecules to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 105.622 µL | 528.111 µL | 1.056 mL | 5.281 mL | 10.562 mL |
5 mM | 21.124 µL | 105.622 µL | 211.245 µL | 1.056 mL | 2.112 mL |
10 mM | 10.562 µL | 52.811 µL | 105.622 µL | 528.111 µL | 1.056 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcal_520.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fcal_520.png&w=2048&q=50)
Spectral properties
Excitation (nm) | 492 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Product Family
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Cal-520®, AM | 492 | 515 | 0.751 |
Cal-520L™ maleimide | 492 | 515 | 0.751 |
Citations
View all 93 citations: Citation Explorer
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
Authors: Schultz, Simon R and Copel, undefined and , Caroline S and Foust, Am and a J , undefined and Quicke, Peter and Schuck, Renaud
Journal: Proceedings of the IEEE (2017): 139--157
High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging
Authors: Daily, Neil J and Du, Zhong-Wei and Wakatsuki, Tetsuro
Journal: ASSAY and Drug Development Technologies (2017)
Authors: Daily, Neil J and Du, Zhong-Wei and Wakatsuki, Tetsuro
Journal: ASSAY and Drug Development Technologies (2017)
HTS-Compatible Voltage-and Ca 2+-Sensitive Dye Recordings from hiPSC-Derived Cardiomyocytes Using the Hamamatsu FDSS Systems
Authors: Kettenhofen, Ralf
Journal: Stem Cell-Derived Models in Toxicology (2017): 135--152
Authors: Kettenhofen, Ralf
Journal: Stem Cell-Derived Models in Toxicology (2017): 135--152
Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes
Authors: Servin-Vences, M Rocio and Moroni, Mirko and Lewin, Gary R and Poole, Kate
Journal: eLife (2017): e21074
Authors: Servin-Vences, M Rocio and Moroni, Mirko and Lewin, Gary R and Poole, Kate
Journal: eLife (2017): e21074
Synergistic drug combination effectively blocks Ebola virus infection
Authors: Sun, Wei and He, Shihua and Martínez-Romero, Carles and Kouznetsova, Jennifer and Tawa, Gregory and Xu, Miao and Shinn, Paul and Fisher, Ethan G and Long, Yan and Motabar, Omid and others, undefined
Journal: Antiviral Research (2017): 165--172
Authors: Sun, Wei and He, Shihua and Martínez-Romero, Carles and Kouznetsova, Jennifer and Tawa, Gregory and Xu, Miao and Shinn, Paul and Fisher, Ethan G and Long, Yan and Motabar, Omid and others, undefined
Journal: Antiviral Research (2017): 165--172
Properties of synchronous spontaneous Ca2+ transients in the mural cells of rat rectal arterioles
Authors: Mitsui, Retsu and Hashitani, Hikaru
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--14
Authors: Mitsui, Retsu and Hashitani, Hikaru
Journal: Pflügers Archiv-European Journal of Physiology (2017): 1--14
A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse
Authors: Pan, Libiao and Yang, Junhua and Yang, Qian and Wang, Xiaomeng and Zhu, Liya and Liu, Yali and Lou, Huifang and Xu, Chou and Shen, Ying and Wang, Hao
Journal: Frontiers in molecular neuroscience (2017)
Authors: Pan, Libiao and Yang, Junhua and Yang, Qian and Wang, Xiaomeng and Zhu, Liya and Liu, Yali and Lou, Huifang and Xu, Chou and Shen, Ying and Wang, Hao
Journal: Frontiers in molecular neuroscience (2017)
Retinal origin of direction selectivity in the superior colliculus
Authors: Shi, Xuefeng and Barchini, Jad and Ledesma, Hector Acaron and Koren, David and Jin, Yanjiao and Liu, Xiaorong and Wei, Wei and Cang, Jianhua
Journal: Nature Neuroscience (2017)
Authors: Shi, Xuefeng and Barchini, Jad and Ledesma, Hector Acaron and Koren, David and Jin, Yanjiao and Liu, Xiaorong and Wei, Wei and Cang, Jianhua
Journal: Nature Neuroscience (2017)
Bidirectional communication between sensory neurons and osteoblasts in an in vitro co-culture system
Authors: Kodama, Daisuke and Hirai, Takao and Kondo, Hisataka and Hamamura, Kazunori and Togari, Akifumi
Journal: FEBS Letters (2017)
Authors: Kodama, Daisuke and Hirai, Takao and Kondo, Hisataka and Hamamura, Kazunori and Togari, Akifumi
Journal: FEBS Letters (2017)
Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools
Authors: Daily, Neil J and Santos, Radleigh and Vecchi, Joseph and Kemanli, Pinar and Wakatsuki, Tetsuro
Journal: Journal of evolving stem cell research (2017): 1
Authors: Daily, Neil J and Santos, Radleigh and Vecchi, Joseph and Kemanli, Pinar and Wakatsuki, Tetsuro
Journal: Journal of evolving stem cell research (2017): 1
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
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Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?