ATTO 425 Biotin Conjugate

• Ex/Em: 438/484 nm
• Extinction coefficient: 45,000 cm^-1M^-1
• Reactive group: biotin
• High-Affinity Binding: Provides strong, reliable attachment to avidin and streptavidin, ideal for sensitive detection and robust biotin-streptavidin assays
• Superior Brightness & Stability: Delivers robust, steady fluorescence for sensitive applications
• Enhanced Signal Separation: Minimal excitation and emission overlap reduces background interference

ATTO 425 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, excellent photostability, and low molecular weight. The dye exhibits moderate hydrophilicity and has an optimal excitation range of 405-455 nm. ATTO 425 is particularly suited for applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a range of other biological assays.

ATTO 425 Biotin conjugates are utilized in diverse applications such as ELISA, immunohistochemistry, in situ hybridization, and flow cytometry for the detection of streptavidin, avidin, or extravidin-conjugates. In biotechnology, biotin is a critical reagent for protein detection, purification, and immobilization due to its strong binding affinity to streptavidin and avidin. The minimal size of the biotin moiety generally preserves the functional integrity of biotinylated proteins. ATTO 425 biotin consists of a biotinyl group, a spacer arm, and an ATTO 425 dye.