ATTO 390 Biotin Conjugate

• Ex/Em: 390/475 nm
• Extinction coefficient: 24,000 cm^-1M^-1
• Reactive group: biotin
• High-Affinity Binding: Provides strong, reliable attachment to avidin, streptavidin, and extravidin for sensitive detection and robust biotin-streptavidin assays
• Enhanced Brightness & Stability: Delivers robust, steady fluorescence for sensitive applications
• Large Stokes Shift: Enables effective signal separation, reducing background interference

ATTO 390 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, good photostability, and low molecular weight, making it a valuable tool in various scientific applications. The dye exhibits moderate hydrophilicity and has an optimal excitation range of 360-410 nm, aligning well with the emission lines of mercury arc lamps at 365 nm and 405 nm. ATTO 390 is particularly suited for single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a range of other biological assays.

ATTO 390 Biotin conjugates are utilized in diverse applications such as ELISA, immunohistochemistry, in situ hybridization, and flow cytometry for the detection of streptavidin, avidin, or extravidin-conjugates. In biotechnology, biotin is a critical reagent for protein detection, purification, and immobilization due to its strong binding affinity to streptavidin and avidin. The minimal size of the biotin moiety generally preserves the functional integrity of biotinylated proteins. ATTO 390 biotin consists of a biotinyl group, a spacer arm, and an ATTO 390 dye.