AF532 PEG4 biotin conjugate

AF532 PEG4 biotin conjugate contains Alexa Fluor 532 fluorophore that is well excited by the common 532 nm laser. It demonstrates excellent avidin binding and strong orange fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. AF532 PEG4 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and short wavelength emission. AF532 PEG4 biotin conjugate is a water-soluble reagent and has pH independent fluorescence from pH 4 to pH 10. The flexible PEG4 spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Page updated on December 17, 2024