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7-AAD
7-Aminoactinomycin D; CAS 7240-37-1
7-Amino actinomycin D (7-AAD) is a non-permeant dye that can be used to identify non-viable cells. Cells with damaged plasma membranes or with impaired/no cell metabolism are unable to prevent the dye from entering the cell. Once inside the cell, the dyes bind to intracellular DNA producing highly fluorescent adducts which identify the cells as "non-viable". It is widely used in flow cytometry. 7-AAD is excited by the 488 nm laser line of an argon laser with fluorescence detected above 650 nm. Although the emission intensity of 7-AAD is lower than that of PI, the longer wavelength emission may make it more useful for multiplexing assays in combination with other 488 nm-excited fluorochromes such as FITC and PE.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 78.714 µL | 393.568 µL | 787.135 µL | 3.936 mL | 7.871 mL |
| 5 mM | 15.743 µL | 78.714 µL | 157.427 µL | 787.135 µL | 1.574 mL |
| 10 mM | 7.871 µL | 39.357 µL | 78.714 µL | 393.568 µL | 787.135 µL |
Molarity calculator
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Citations
View all 14 citations: Citation Explorer
Propofol elicits apoptosis and attenuates cell growth in esophageal cancer cell lines
Authors: Zhou, Rui and Konishi, Yuko and Zhang, Ailing and Nishiwaki, Kimitoshi
Journal: Nagoya J. Med. Sci (2023): 579--591
Authors: Zhou, Rui and Konishi, Yuko and Zhang, Ailing and Nishiwaki, Kimitoshi
Journal: Nagoya J. Med. Sci (2023): 579--591
Reconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants
Authors: Aabling, Rasmus Roost and Alstrup, Toke and Kj{\ae}r, Emma Mader and Poulsen, Kristine Juul and Pedersen, Jonas Oute and Revenfeld, Anne Louise and M{\o}ller, Bjarne Kuno and Eijken, Marco
Journal: Regenerative Therapy (2023): 67--75
Authors: Aabling, Rasmus Roost and Alstrup, Toke and Kj{\ae}r, Emma Mader and Poulsen, Kristine Juul and Pedersen, Jonas Oute and Revenfeld, Anne Louise and M{\o}ller, Bjarne Kuno and Eijken, Marco
Journal: Regenerative Therapy (2023): 67--75
Rapid fabrication by digital light processing 3D printing of a SlipChip with movable ports for local delivery to ex vivo organ cultures
Authors: Catterton, Megan and Ball, Alexander and Pompano, Rebecca
Journal: (2021)
Authors: Catterton, Megan and Ball, Alexander and Pompano, Rebecca
Journal: (2021)
Development and improved fabrication of microfluidic devices for local delivery to investigate properties of healthy and inflamed lymph node tissue ex vivo slices.
Authors: Catterton, Megan Ann
Journal: (2021)
Authors: Catterton, Megan Ann
Journal: (2021)
Labelling primary immune cells using bright blue fluorescent nanoparticles
Authors: Belanger, Maura C and Zhuang, Meng and Ball, Alexander G and Richey, Kristen H and DeRosa, Christopher A and Fraser, Cassandra L and Pompano, Rebecca R
Journal: Biomaterials science (2020): 1897--1909
Authors: Belanger, Maura C and Zhuang, Meng and Ball, Alexander G and Richey, Kristen H and DeRosa, Christopher A and Fraser, Cassandra L and Pompano, Rebecca R
Journal: Biomaterials science (2020): 1897--1909
References
View all 11 references: Citation Explorer
A study of interaction of 7-aminoactinomycin D with DNA by fluorescence correlation spectroscopy
Authors: Kovalev AE, Iakovenko AA, Vekshin NL.
Journal: Biofizika (2004): 1030
Authors: Kovalev AE, Iakovenko AA, Vekshin NL.
Journal: Biofizika (2004): 1030
Phagocytosis of apoptotic cells assessed by flow cytometry using 7-Aminoactinomycin D
Authors: Gaforio JJ, Serrano MJ, Algarra I, Ortega E, Alvarez de Cienfuegos G.
Journal: Cytometry (2002): 8
Authors: Gaforio JJ, Serrano MJ, Algarra I, Ortega E, Alvarez de Cienfuegos G.
Journal: Cytometry (2002): 8
Multiparametric flow cytometric analysis of biochemical and functional events associated with apoptosis and oncosis using the 7-aminoactinomycin D assay
Authors: Lecoeur H, de Oliveira-Pinto LM, Gougeon ML.
Journal: J Immunol Methods (2002): 81
Authors: Lecoeur H, de Oliveira-Pinto LM, Gougeon ML.
Journal: J Immunol Methods (2002): 81
Accessibility of 7-aminoactinomycin D to lymphocyte nuclei after paraformaldehyde fixation
Authors: Linden E, Skoglund P, Rundquist I.
Journal: Cytometry (1997): 92
Authors: Linden E, Skoglund P, Rundquist I.
Journal: Cytometry (1997): 92
High affinity binding of 7-aminoactinomycin D and 4',6-diamidino-2-phenylindole to human neutrophilic granulocytes and lymphocytes
Authors: Loborg H, Linden E, Lonn A, Skoglund P, Rundquist I.
Journal: Cytometry (1995): 296
Authors: Loborg H, Linden E, Lonn A, Skoglund P, Rundquist I.
Journal: Cytometry (1995): 296
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