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6-ROX alkyne

6-ROX alkyne is an excellent azide-reactive fluorescent reagent for labeling alkyne-containing biological molecules through the well known click chemistry. It has the spectral properties identical to those of other 6-ROX conjugates, thus all the instrument settings of the 6-ROX conjugates would be applicable to the applications of 6-ROX alkyne conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROX alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM174.929 µL874.646 µL1.749 mL8.746 mL17.493 mL
5 mM34.986 µL174.929 µL349.858 µL1.749 mL3.499 mL
10 mM17.493 µL87.465 µL174.929 µL874.646 µL1.749 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Alkyne493517830000.178
6-HEX alkyne533559730000.15
6-TET alkyne521542730000.13
6-JOE alkyne520545750001-
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*578604820000.168
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*578604820000.168
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*578604820000.168
6-TAMRA alkyne552578900000.178
6-NED alkyne545567740001-
6-VIC Alkyne526543--
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References

View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Page updated on October 28, 2024

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Physical properties

Molecular weight

571.66

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.168

Extinction coefficient (cm -1 M -1)

82000

Excitation (nm)

578

Emission (nm)

604

Quantum yield

0.941

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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