6-TET alkyne

6-TET is widely used in nucleic acid sequencing and related research. 6-TET alkyne provides a convenient method for labeling azide-modified oligos throught the well-known click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	181.436 µL	907.178 µL	1.814 mL	9.072 mL	18.144 mL
5 mM	36.287 µL	181.436 µL	362.871 µL	1.814 mL	3.629 mL
10 mM	18.144 µL	90.718 µL	181.436 µL	907.178 µL	1.814 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM Alkyne	493	517	83000	0.178
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]	521	542	73000	0.13
6-HEX alkyne	533	559	73000	0.15
6-JOE alkyne	520	545	75000¹	-
6-TAMRA alkyne	552	578	90000	0.178
6-ROX alkyne	578	604	82000	0.168
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] CAS#: 877049-90-6	521	542	73000	0.13
6-NED alkyne	545	567	74000¹	-
6-VIC Alkyne	526	543	-	-

References

View all 12 references: Citation Explorer

Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098

Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947

Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016

Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61

Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91

Page updated on October 28, 2024

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Physical properties

Molecular weight	551.16
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.13
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	521
Emission (nm)	542

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501