6-TET alkyne

6-TET is widely used in nucleic acid sequencing and related research. 6-TET alkyne provides a convenient method for labeling azide-modified oligos throught the well-known click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TET alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	181.436 µL	907.178 µL	1.814 mL	9.072 mL	18.144 mL
5 mM	36.287 µL	181.436 µL	362.871 µL	1.814 mL	3.629 mL
10 mM	18.144 µL	90.718 µL	181.436 µL	907.178 µL	1.814 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM Alkyne	493	517	83000	0.178
6-TET, SE [6-Carboxy-2',4,7',7-tetrachlorofluorescein, succinimidyl ester]	521	542	73000	0.13
6-HEX alkyne	533	559	73000	0.15
6-JOE alkyne	520	545	75000¹	-
6-TAMRA alkyne	552	578	90000	0.178
6-ROX alkyne	578	604	82000	0.168
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] CAS#: 877049-90-6	521	542	73000	0.13
6-NED alkyne	545	567	74000¹	-
6-VIC Alkyne	526	543	-	-

References

View all 12 references: Citation Explorer

Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis
Authors: Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: Mol Vis (2006): 1098

Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: Plant J (2004): 947

Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: J Clin Microbiol (2003): 4016

Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61

Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: Simeonov A, Nikiforov TT.
Journal: Nucleic Acids Res (2002): e91

Page updated on December 17, 2024

Ordering information

Price
Unit size
Catalog Number	245
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Request quotation

Physical properties

Molecular weight	551.16
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.13
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	521
Emission (nm)	542

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501