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6-JOE alkyne
6-JOE is widely used in nucleic acid sequencing and related research. 6-JOE alkyne provides a convenient method for labeling azide-modified oligos throught the well-known click chemistry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-JOE alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 184.393 µL | 921.965 µL | 1.844 mL | 9.22 mL | 18.439 mL |
| 5 mM | 36.879 µL | 184.393 µL | 368.786 µL | 1.844 mL | 3.688 mL |
| 10 mM | 18.439 µL | 92.196 µL | 184.393 µL | 921.965 µL | 1.844 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-FAM Alkyne | 493 | 517 | 83000 | 0.178 |
| 6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3* | 520 | 545 | 750001 | - |
| 6-HEX alkyne | 533 | 559 | 73000 | 0.15 |
| 6-TET alkyne | 521 | 542 | 73000 | 0.13 |
| 6-TAMRA alkyne | 552 | 578 | 90000 | 0.178 |
| 6-ROX alkyne | 578 | 604 | 82000 | 0.168 |
| 6-JOE Phosphoramidite | 520 | 545 | 750001 | - |
| 6-NED alkyne | 545 | 567 | 740001 | - |
| 6-VIC Alkyne | 526 | 543 | - | - |
References
View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058
Page updated on November 20, 2024
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