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6-ROX azide
6-ROX azide is an excellent alkyne-reactive fluorescent reagent for labeling alkyne-containing biological molecules through the well known click chemistry. It has the spectral properties identical to those of other 6-ROX conjugates, thus all the instrument settings of the 6-ROX conjugates would be applicable to the applications of 6-ROX azide conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROX azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 165.926 µL | 829.628 µL | 1.659 mL | 8.296 mL | 16.593 mL |
| 5 mM | 33.185 µL | 165.926 µL | 331.851 µL | 1.659 mL | 3.319 mL |
| 10 mM | 16.593 µL | 82.963 µL | 165.926 µL | 829.628 µL | 1.659 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| 6-FAM Azide | 493 | 517 | 83000 | 0.178 |
| 6-HEX azide | 533 | 559 | 73000 | 0.15 |
| 6-TET azide | 521 | 542 | 73000 | 0.13 |
| 6-JOE azide | 520 | 545 | 750001 | - |
| 5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8* | 578 | 604 | 82000 | 0.168 |
| 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* | 578 | 604 | 82000 | 0.168 |
| 6-ROX glycine *25 uM fluorescence reference solution for PCR reactions* | 578 | 604 | 82000 | 0.168 |
| 6-TAMRA azide | 552 | 578 | 90000 | 0.178 |
| 6-NED azide | 545 | 567 | 740001 | - |
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References
View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
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