Which membrane is preferred for fluorescence-based detection in western blotting?

For fluorescence-based detection in western blotting, low-fluorescence PVDF membranes are generally preferred over nitrocellulose membranes. This is because nitrocellulose membranes tend to exhibit higher levels of autofluorescence. Low-fluorescence PVDF membranes also tend to offer greater sensitivity, potentially due to their enhanced protein binding capabilities.