Can I strip and reprobe PVDF and nitrocellulose membranes?

Nitrocellulose membranes are easily hydrated and commonly used in wet or semi-dry transfers. However, since they are fragile, it limits their compatibility with stripping and re-probing techniques due to potential damage to the membrane. On the other hand, PVDF membranes are more robust, making them suitable for stripping and reprobing. PVDF membranes bind proteins through hydrophobic interactions and have a higher protein-binding capacity compared to other substrates, allowing them to bind approximately 150μg/cm2 of protein.