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AAT Bioquest

What is a stacking gel?

Posted July 31, 2024


Answer

A stacking gel is a type of low concentration polyacrylamide gel used in a laboratory technique known as SDS-PAGE (sodium dodecyl polyacrylamide gel electrophoresis). It contains 4% polyacrylamide, resulting in larger pore size. The pH of this gel is maintained at 6.8.

Additional resources

Urine protein quantification in stacking gel by SDS-PAGE

Gel Electrophoresis

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