What are the advantages of using fluorescence to detect western blots over chemiluminescence?

There are several advantages of using fluorescence to detect western blots over chemiluminescence. Firstly, it allows for multiplexing, which is time efficient and also conserves sample material. Fluorescent signals are also typically more stable over time compared to chemiluminescent signals. This stability allows for longer exposure times and more consistent results, reducing variability between experiments. Another advantage is that unlike chemiluminescent detection, fluorescent detection eliminates the need to strip and reprobe the blot when looking at multiple targets. Fluorescent detection also allows for more accurate measurement of protein concentrations across a wider range.