What are the advantages of polyclonal antibodies?
Posted October 7, 2022
Answer
Polyclonal antibodies (pAbs) are a heterogeneous mixture of multiple antibodies that are typically derived by different B-cell clones of an animal. They are produced by the inoculation of a suitable mammal, such as a rabbit, goat or mouse. Larger mammals are often preferred as the amount of serum derived is greater. Advantages of polyclonal antibodies include:
- Relatively quicker and less expensive to produce
- Have a higher sensitivity for detecting proteins in low quantities
- Offer wider options for producing antibodies in different animals
- Higher overall antibody affinity against the antigen because of its ability to recognize and bind to multiple epitopes
- Faster binding to the target antigen because of higher antibody affinity
- Higher ability to capture the protein of interest
- Relatively simple to purify using affinity chromatography techniques
- Easy to couple with antibody labels
- Does not affect binding capability
Additional resources
Overlooked benefits of using polyclonal antibodies
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