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AAT Bioquest

How does colloidal Coomassie work?

Posted August 14, 2024


Answer

Colloidal Coomassie is a variation of traditional Coomassie staining that uses a modified Coomassie dye, specifically G-250 instead of R-250. Since the colloidal dye doesn't penetrate the gel, there's no requirement for destaining. However, destaining can be done to enhance the contrast between the protein bands and the background. Colloidal Coomassie stains can be prepared to stain proteins effectively within 1 hour and only need water for the process.

Additional resources

Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels

Gelite™ Safe DNA Gel Stain *10,000X Water Solution*