What are the applications of Coomassie blue?

One application of Coomassie blue includes its use in electrophoresis. Proteins separated using PAGE can be visualized using Coomassie blue, as well as fluorescent or silver staining methods. Coomassie blue staining is favored in many labs because it is quick, easy, cost-effective, and doesn't require fixation. It is also more compatible with mass spectrometry compared to other staining techniques. Another common application involves its use in assays. Coomassie blue is commonly used in protein quantification assays, such as the Bradford assay. In these assays, the dye binds to protein molecules, allowing their concentration to be measured.