What markers can I use for investigating microglial activation in flow cytometry?

Posted May 9, 2020

Antibodies and Proteomics

Antibody and Protein Labeling

Cluster of Differentiation (CD Antibodies)

Flow Cytometry Reagents

Immunohistochemistry (IHC)

Answer

Some markers that can be used to studying microglial activation include CD40, CD45, CD68, CD86, and MHC-II.

Additional resources

Hopperton, K. E., Mohammad, D., Trépanier, M. O., Giuliano, V., & Bazinet, R. P. (2018). Markers of microglia in post-mortem brain samples from patients with Alzheimer’s disease: a systematic review. Molecular psychiatry, 23(2), 177-198.

Ponomarev, E. D., Shriver, L. P., & Dittel, B. N. (2006). CD40 expression by microglial cells is required for their completion of a two-step activation process during central nervous system autoimmune inflammation. The Journal of Immunology, 176(3), 1402-1410.

Cluster of Differentiation (CD Antibodies)

iFluor™ A7 Anti-human CD45 Antibody

iFluor® A7 Anti-human CD40 Antibody *G28.5*

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