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AAT Bioquest

How to circumvent the non specific binding of monoclonal Antibody?

Posted April 17, 2020


Answer

In order to circumvent the non-specific binding of monoclonal antibodies, one can use a highly specific antibody, lower the dilution of the antibody, shorten the incubation time, and incorporate a thorough washing step after incubation with the primary.

Additional resources

CD Antibodies