How do you increase immunoprecipitation efficiency?

These five tips will help increase immunoprecipitation efficiency:

• Use the highest protein concentration possible. The higher the protein concentration, the higher the IP yield.
• Choose the right antibody with a higher binding affinity. The higher the binding affinity, the higher the protein yield.
• Use the smallest volume possible. A smaller volume keeps the protein concentration high, increasing the binding affinity. Immunoprecipitation is most efficient at small volumes.
• Use a shorter wash time. This prevents excessive leaching of the bound protein, increasing the pull-down yield.
• Use specific, well-defined affinity matrices to get reliable, reproducible results.