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AAT Bioquest

How are agarose gels with ethidium bromide decontaminated?

Posted December 1, 2021


Answer

The correct method used for decontaminating agarose gels with ethidium bromide (EtBr) depends on the amount of EtBr in the gel.

Agarose gels containing trace amounts of less than 0.1% ethidium bromide can be wrapped in a plastic bag and discarded in the trash.

 Gels containing more than 0.1% must be collected in a double-bagged sealed container and placed in an appropriately labeled bin before requesting a toxic waste removal.

Additional resources

Decontamination of ethidium bromide spills

Helixyte™ Green Nucleic Acid Gel Stain *10,000X DMSO Solution*