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Z-VDVAD-aminoluciferin
Z-VDVAD-aminoluciferin is a luminogenic substrate that measures caspase-2 activity in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. The pentapeptide sequence VDVAD in this substrate is specific for protease cleavage by caspase-2. Following caspase-2 activation, the VDVAD peptide is cleaved just after the second aspartic acid residue (D), releasing aminoluciferin, which serves as a luminescent substrate for the luciferase reaction. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-2 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Z-DEVD-aminoluciferin | 362 | 499 | 18000 |
| Z-LETD-aminoluciferin | 362 | 499 | 18000 |
| Z-LEHD-aminoluciferin | 362 | 499 | 18000 |
| Z-IETD-aminoluciferin | 362 | 499 | 18000 |
| Z-VEID-aminoluciferin | 362 | 499 | 18000 |
| Z-ATAD-aminoluciferin | 362 | 499 | 18000 |
References
View all 50 references: Citation Explorer
Chemoproteomics Identifies State-Dependent and Proteoform-Selective Caspase-2 Inhibitors.
Authors: Castellón, José O and Ofori, Samuel and Burton, Nikolas R and Julio, Ashley R and Turmon, Alexandra C and Armenta, Ernest and Sandoval, Carina and Boatner, Lisa M and Takayoshi, Evan E and Faragalla, Marina and Taylor, Cameron and Zhou, Ann L and Tran, Ky and Shek, Jeremy and Yan, Tianyang and Desai, Heta S and Fregoso, Oliver I and Damoiseaux, Robert and Backus, Keriann M
Journal: Journal of the American Chemical Society (2024): 14972-14988
Authors: Castellón, José O and Ofori, Samuel and Burton, Nikolas R and Julio, Ashley R and Turmon, Alexandra C and Armenta, Ernest and Sandoval, Carina and Boatner, Lisa M and Takayoshi, Evan E and Faragalla, Marina and Taylor, Cameron and Zhou, Ann L and Tran, Ky and Shek, Jeremy and Yan, Tianyang and Desai, Heta S and Fregoso, Oliver I and Damoiseaux, Robert and Backus, Keriann M
Journal: Journal of the American Chemical Society (2024): 14972-14988
Validation of the Intermolecular Disulfide Bond in Caspase-2.
Authors: Amason, Megan E and Li, Lupeng and Harvest, Carissa K and Lacey, Carolyn A and Miao, Edward A
Journal: Biology (2024)
Authors: Amason, Megan E and Li, Lupeng and Harvest, Carissa K and Lacey, Carolyn A and Miao, Edward A
Journal: Biology (2024)
Caspase-2 is essential for proliferation and self-renewal of nucleophosmin-mutated acute myeloid leukemia.
Authors: Sakthivel, Dharaniya and Brown-Suedel, Alexandra N and Lopez, Karla E and Salgar, Suruchi and Coutinho, Luiza E and Keane, Francesca and Huang, Shixia and Sherry, Kenneth Mc and Charendoff, Chloé I and Dunne, Kevin P and Robichaux, Dexter J and Vargas-Hernández, Alexander and Le, BaoChau and Shin, Crystal S and Carisey, Alexandre F and Poreba, Marcin and Flanagan, Jonathan M and Bouchier-Hayes, Lisa
Journal: Science advances (2024): eadj3145
Authors: Sakthivel, Dharaniya and Brown-Suedel, Alexandra N and Lopez, Karla E and Salgar, Suruchi and Coutinho, Luiza E and Keane, Francesca and Huang, Shixia and Sherry, Kenneth Mc and Charendoff, Chloé I and Dunne, Kevin P and Robichaux, Dexter J and Vargas-Hernández, Alexander and Le, BaoChau and Shin, Crystal S and Carisey, Alexandre F and Poreba, Marcin and Flanagan, Jonathan M and Bouchier-Hayes, Lisa
Journal: Science advances (2024): eadj3145
Dissecting caspase-2-mediated cell death: from intrinsic PIDDosome activation to chemical modulation.
Authors: Zeng, Mengxue and Wang, Kun and Wu, Qingcui and Ding, Jingjin and Xie, Dan and Qi, Xiangbing and Shao, Feng
Journal: Protein & cell (2024)
Authors: Zeng, Mengxue and Wang, Kun and Wu, Qingcui and Ding, Jingjin and Xie, Dan and Qi, Xiangbing and Shao, Feng
Journal: Protein & cell (2024)
Caspase-2 protects against ferroptotic cell death.
Authors: Dawar, Swati and Benitez, Mariana C and Lim, Yoon and Dite, Toby A and Yousef, Jumana M and Thio, Niko and Garciaz, Sylvain and Jackson, Thomas D and Milne, Julia V and Dagley, Laura F and Phillips, Wayne A and Kumar, Sharad and Clemons, Nicholas J
Journal: Cell death & disease (2024): 182
Authors: Dawar, Swati and Benitez, Mariana C and Lim, Yoon and Dite, Toby A and Yousef, Jumana M and Thio, Niko and Garciaz, Sylvain and Jackson, Thomas D and Milne, Julia V and Dagley, Laura F and Phillips, Wayne A and Kumar, Sharad and Clemons, Nicholas J
Journal: Cell death & disease (2024): 182
Page updated on December 17, 2024
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