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Z-LETD-aminoluciferin
Z-LETD-aminoluciferin is a luminogenic substrate that measures caspase-8 activity in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. The tetrapeptide sequence LETD in this substrate is specific for protease cleavage by caspase-8. Following caspase-8 activation, the LETD peptide is cleaved just after the aspartic acid residue (D), releasing aminoluciferin, a luminescent substrate for the luciferase reaction. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-8 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Z-DEVD-aminoluciferin | 362 | 499 | 18000 |
| Z-LEHD-aminoluciferin | 362 | 499 | 18000 |
| Z-IETD-aminoluciferin | 362 | 499 | 18000 |
| Z-VDVAD-aminoluciferin | 362 | 499 | 18000 |
| Z-VEID-aminoluciferin | 362 | 499 | 18000 |
| Z-ATAD-aminoluciferin | 362 | 499 | 18000 |
References
View all 50 references: Citation Explorer
The TRIF-RIPK1-Caspase-8 signalling in the regulation of TLR4-driven gene expression.
Authors: Zhang, Chengyang and Zhou, Yang and Xi, Shuangtong and Han, Danlin and Wang, Ziyu and Zhu, Jingwen and Cai, Yizhe and Zhang, Haifeng and Jin, Ge and Mi, Yang
Journal: Immunology (2024): 566-576
Authors: Zhang, Chengyang and Zhou, Yang and Xi, Shuangtong and Han, Danlin and Wang, Ziyu and Zhu, Jingwen and Cai, Yizhe and Zhang, Haifeng and Jin, Ge and Mi, Yang
Journal: Immunology (2024): 566-576
Arginine methylation of caspase-8 controls life/death decisions in extrinsic apoptotic networks.
Authors: Wohlfromm, Fabian and Ivanisenko, Nikita V and Pietkiewicz, Sabine and König, Corinna and Seyrek, Kamil and Kähne, Thilo and Lavrik, Inna N
Journal: Oncogene (2024): 1955-1971
Authors: Wohlfromm, Fabian and Ivanisenko, Nikita V and Pietkiewicz, Sabine and König, Corinna and Seyrek, Kamil and Kähne, Thilo and Lavrik, Inna N
Journal: Oncogene (2024): 1955-1971
Gancao decoction attenuates hepatic necroptosis via activating caspase 8 in cholestatic liver injury.
Authors: Zou, Bin and Zhang, Shuang and Li, Fengling and Weng, Fengyi and Zhao, Jing and Jin, Jingyi and Yan, Dongming and Xu, Xiaoqing and Chen, Gaofeng and Liu, Chenghai and Yao, Chengzeng and Li, Yue and Qiu, Furong
Journal: Journal of ethnopharmacology (2024): 117909
Authors: Zou, Bin and Zhang, Shuang and Li, Fengling and Weng, Fengyi and Zhao, Jing and Jin, Jingyi and Yan, Dongming and Xu, Xiaoqing and Chen, Gaofeng and Liu, Chenghai and Yao, Chengzeng and Li, Yue and Qiu, Furong
Journal: Journal of ethnopharmacology (2024): 117909
Caspase-8-mediated inflammation but not apoptosis drives death of retinal ganglion cells and loss of visual function in glaucomaa.
Authors: Guo, Yinjie and Verma, Bhupender and Shrestha, Maleeka and Marshak-Rothstein, Ann and Gregory-Ksander, Meredith
Journal: Research square (2024)
Authors: Guo, Yinjie and Verma, Bhupender and Shrestha, Maleeka and Marshak-Rothstein, Ann and Gregory-Ksander, Meredith
Journal: Research square (2024)
Caspase-8 activity mediates TNFα production and restricts Coxiella burnetii replication during murine macrophage infection.
Authors: Osbron, Chelsea A and Lawson, Crystal and Hanna, Nolan and Koehler, Heather S and Goodman, Alan G
Journal: Infection and immunity (2024): e0005324
Authors: Osbron, Chelsea A and Lawson, Crystal and Hanna, Nolan and Koehler, Heather S and Goodman, Alan G
Journal: Infection and immunity (2024): e0005324
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