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Z-LEHD-aminoluciferin
Z-LEHD-aminoluciferin is a luminogenic substrate that measures caspase-9 activity in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. The tetrapeptide sequence LEHD in this substrate is specific for protease cleavage by caspase-9. Following caspase-9 activation, the LEHD peptide is cleaved just after the aspartic acid residue (D), releasing aminoluciferin, which serves as a luminescent substrate for the luciferase reaction. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-9 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Z-DEVD-aminoluciferin | 362 | 499 | 18000 |
| Z-LETD-aminoluciferin | 362 | 499 | 18000 |
| Z-IETD-aminoluciferin | 362 | 499 | 18000 |
| Z-VDVAD-aminoluciferin | 362 | 499 | 18000 |
| Z-VEID-aminoluciferin | 362 | 499 | 18000 |
| Z-ATAD-aminoluciferin | 362 | 499 | 18000 |
References
View all 50 references: Citation Explorer
Caspase-9 suppresses metastatic behavior of MDA-MB-231 cells in an adaptive organoid model.
Authors: Falahi, Farzaneh and Akbari-Birgani, Shiva and Mortazavi, Yousef and Johari, Behrooz
Journal: Scientific reports (2024): 15116
Authors: Falahi, Farzaneh and Akbari-Birgani, Shiva and Mortazavi, Yousef and Johari, Behrooz
Journal: Scientific reports (2024): 15116
Roles of Cyt-c/Caspase-9/Caspase-3/Bax/Bcl-2 pathway in Cd-induced testicular injury in rats and the protective effect of quercetin.
Authors: Yu, Wenjing and Zhu, Huali and Huang, Ruxue and Yan, Bingzhao and Xu, Bing and Shi, Yaning and Mao, Junbing and Liu, Zongping and Wang, Jicang
Journal: Toxicon : official journal of the International Society on Toxinology (2024): 107561
Authors: Yu, Wenjing and Zhu, Huali and Huang, Ruxue and Yan, Bingzhao and Xu, Bing and Shi, Yaning and Mao, Junbing and Liu, Zongping and Wang, Jicang
Journal: Toxicon : official journal of the International Society on Toxinology (2024): 107561
Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics.
Authors: Říhová, Kamila and Lapčík, Petr and Veselá, Barbora and Knopfová, Lucia and Potěšil, David and Pokludová, Jana and Šmarda, Jan and Matalová, Eva and Bouchal, Pavel and Beneš, Petr
Journal: Journal of proteome research (2024): 2999-3011
Authors: Říhová, Kamila and Lapčík, Petr and Veselá, Barbora and Knopfová, Lucia and Potěšil, David and Pokludová, Jana and Šmarda, Jan and Matalová, Eva and Bouchal, Pavel and Beneš, Petr
Journal: Journal of proteome research (2024): 2999-3011
A Non-Apoptotic Pattern of Caspase-9/Caspase-3 Activation During Differentiation of Human Embryonic Stem Cells into Cardiomyocytes.
Authors: Ghorbani, Negar and Shiri, Mahshad and Alian, Maedeh and Yaghubi, Roham and Shafaghi, Mojtaba and Hojjat, Hamidreza and Pahlavan, Sara and Davoodi, Jamshid
Journal: Advanced biology (2024): e2400026
Authors: Ghorbani, Negar and Shiri, Mahshad and Alian, Maedeh and Yaghubi, Roham and Shafaghi, Mojtaba and Hojjat, Hamidreza and Pahlavan, Sara and Davoodi, Jamshid
Journal: Advanced biology (2024): e2400026
Chimeric Antigen Receptor T Cell with an Inducible Caspase-9 Suicide Gene Eradicates Uveal Melanoma Liver Metastases via B7-H3 Targeting.
Authors: Ventin, Marco and Cattaneo, Giulia and Arya, Shahrzad and Jia, Jingyu and Gelmi, Maria C and Sun, Yi and Maggs, Luke and Ksander, Bruce R and Verdijk, Robert M and Boland, Genevieve M and Jenkins, Russell W and Haq, Rizwan and Jager, Martine J and Wang, Xinhui and Ryeom, Sandra and Ferrone, Cristina R
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (2024): 3243-3258
Authors: Ventin, Marco and Cattaneo, Giulia and Arya, Shahrzad and Jia, Jingyu and Gelmi, Maria C and Sun, Yi and Maggs, Luke and Ksander, Bruce R and Verdijk, Robert M and Boland, Genevieve M and Jenkins, Russell W and Haq, Rizwan and Jager, Martine J and Wang, Xinhui and Ryeom, Sandra and Ferrone, Cristina R
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (2024): 3243-3258
Page updated on November 21, 2024
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