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Z-ATAD-aminoluciferin
Z-ATAD-aminoluciferin is a luminogenic substrate that measures caspase-12 activity in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. The tetrapeptide sequence ATAD in this substrate is specific for protease cleavage by caspase-12. Following caspase-12 activation, the ATAD peptide is cleaved just after the aspartic acid residue (D), releasing aminoluciferin, which serves as a luminescent substrate for the luciferase reaction. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-12 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Z-DEVD-aminoluciferin | 362 | 499 | 18000 |
| Z-LETD-aminoluciferin | 362 | 499 | 18000 |
| Z-LEHD-aminoluciferin | 362 | 499 | 18000 |
| Z-IETD-aminoluciferin | 362 | 499 | 18000 |
| Z-VDVAD-aminoluciferin | 362 | 499 | 18000 |
| Z-VEID-aminoluciferin | 362 | 499 | 18000 |
References
View all 50 references: Citation Explorer
Caspase-12 is Expressed in Purkinje Neurons and Prevents Psychiatric-Like Behavior in Mice.
Authors: Huang, Lin-Yan and Liu, Yi-Ning and Chen, Jie and Zhu, Hai-Xue and Li, Li-Li and Liang, Zhi-Yan and Song, Jin-Xiu and Li, Yu-Jie and Hu, Zhao-Li and Demon, Dieter and Wullaert, Andy and Wang, Wan and Qi, Su-Hua
Journal: Molecular neurobiology (2024)
Authors: Huang, Lin-Yan and Liu, Yi-Ning and Chen, Jie and Zhu, Hai-Xue and Li, Li-Li and Liang, Zhi-Yan and Song, Jin-Xiu and Li, Yu-Jie and Hu, Zhao-Li and Demon, Dieter and Wullaert, Andy and Wang, Wan and Qi, Su-Hua
Journal: Molecular neurobiology (2024)
Insulin reduces endoplasmic reticulum stress-induced apoptosis by decreasing mitochondrial hyperpolarization and caspase-12 in INS-1 pancreatic β-cells.
Authors: Murata, Nanako and Nishimura, Kana and Harada, Naoki and Kitakaze, Tomoya and Yoshihara, Eiji and Inui, Hiroshi and Yamaji, Ryoichi
Journal: Physiological reports (2024): e16106
Authors: Murata, Nanako and Nishimura, Kana and Harada, Naoki and Kitakaze, Tomoya and Yoshihara, Eiji and Inui, Hiroshi and Yamaji, Ryoichi
Journal: Physiological reports (2024): e16106
IL-17A exacerbates caspase-12-dependent neuronal apoptosis following ischemia through the Src-PLCγ-calpain pathway.
Authors: Wang, Hongyu and Han, Song and Xie, Jinjin and Zhao, Ruixue and Li, Shujuan and Li, Junfa
Journal: Experimental neurology (2024): 114863
Authors: Wang, Hongyu and Han, Song and Xie, Jinjin and Zhao, Ruixue and Li, Shujuan and Li, Junfa
Journal: Experimental neurology (2024): 114863
Cigarette smoking induces the activation of RIP2/caspase-12/NF-κB axis in oral squamous cell carcinoma.
Authors: Qian, Yajie and Wang, Wenmei and Chen, Deyan and Zhu, Yanan and Wang, Yong and Wang, Xiang
Journal: PeerJ (2022): e14330
Authors: Qian, Yajie and Wang, Wenmei and Chen, Deyan and Zhu, Yanan and Wang, Yong and Wang, Xiang
Journal: PeerJ (2022): e14330
[Effect of acupuncture on expression of endoplasmic reticulum Ca2+ and Caspase-12 protein in hippocampal neurons of rats with convulsive brain injury].
Authors: Zhang, Hui and Ma, Yun and Wu, Xu and Liu, Zhen-Bang and Wu, Sheng-Bing and Ang, Wen-Ping and Yang, Fan
Journal: Zhen ci yan jiu = Acupuncture research (2022): 673-7
Authors: Zhang, Hui and Ma, Yun and Wu, Xu and Liu, Zhen-Bang and Wu, Sheng-Bing and Ang, Wen-Ping and Yang, Fan
Journal: Zhen ci yan jiu = Acupuncture research (2022): 673-7
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