XFD488 goat anti-rabbit IgG (H+L) Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488

Name: XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
Brand: AAT Bioquest
SKU: 16398
Price: 170 USD
Availability: InStock

XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher). Our goat anti-rabbit IgG whole antibodies have been cross-adsorbed against human IgG and human serum prior to conjugation to minimize cross-reactivity. This XFD488 labeled-goat anti-rabbit IgG conjugate is prepared by the reaction of cross-adsorbed goat anti-rabbit IgG whole antibody with XFD488 NHS ester, which has the same molecule as Alexa Fluor® 488 NHS ester. Each conjugate has typically 4-6 fluorophores per IgG molecule. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 goat anti-mouse IgG (H+L) Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.3	0.11
XFD350 goat anti-rabbit IgG (H+L) Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350	343	441	19000	-	0.25	0.19
XFD594 goat anti-rabbit IgG (H+L) Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594	590	618	92000	0.66¹	0.43	0.56
FITC goat anti-rabbit IgG (H+L)	491	516	73000	0.92	-	0.35
Cy3® goat anti-rabbit IgG (H+L)	555	569	150000¹	0.15¹	0.07	0.073
Cy5® goat anti-rabbit IgG (H+L)	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy7® goat anti-rabbit IgG (H+L)	756	779	250000	0.3	0.05	0.036
FITC goat anti-rabbit IgG (H+L) Cross Adsorbed	491	516	73000	0.92	-	0.35
Cy3® goat anti-rabbit IgG (H+L) Cross Adsorbed	555	569	150000¹	0.15¹	0.07	0.073
Cy5® goat anti-rabbit IgG (H+L) Cross Adsorbed	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy7® goat anti-rabbit IgG (H+L) Cross Adsorbed	756	779	250000	0.3	0.05	0.036
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Citations

View all 6 citations: Citation Explorer

Hippo signaling pathway regulates Ebola virus transcription and egress
Authors: Liang, Jingjing and Djurkovic, Marija A and Leavitt, Carson G and Shtanko, Olena and Harty, Ronald N
Journal: Nature Communications (2024): 6953

Cell-specific Nav1. 6 knockdown reduced astrocyte-derived A$\beta$ by reverse Na+-Ca2+ transporter-mediated autophagy in alzheimer-like mice
Authors: Wang, Xin and Wu, Wei and Yang, Guang and Yang, Xue-Wei and Ma, Xu and Zhu, Dan-Dan and Ahmad, Kabir and Khan, Khizar and Wang, Ying-Zi and Sui, Ao-Ran and others,
Journal: Journal of Advanced Research (2024)

Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)

Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Ma, Junjun and Zhao, Jingkun and Lu, Jun and Wang, Puxiongzhi and Feng, Hao and Zong, Yaping and Ou, Baochi and Zheng, Minhua and Lu, Aiguo
Journal: Tumor Biology (2016): 1--12

Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury
Authors: Shi, W and Huang, CJ and Xu, XD and Jin, GH and Huang, RQ and Huang, JF and Chen, YN and Ju, SQ and Wang, Y and Shi, YW and others, undefined
Journal: Acta Biomaterialia (2016): 247--261

References

View all 191 references: Citation Explorer

Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728

Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133

Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439

Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126

Page updated on December 3, 2024

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Catalog Number	16398
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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.3
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501