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Tide Quencher™ 2 acid [TQ2 acid]
TQ2 is designed to be a superior quencher to FAM, HEX, TET, JOE, TF2 and rhodamine 6G. TQ2 has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ2 product is primarily used for labeling peptides.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 2 acid [TQ2 acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 261.452 µL | 1.307 mL | 2.615 mL | 13.073 mL | 26.145 mL |
| 5 mM | 52.29 µL | 261.452 µL | 522.903 µL | 2.615 mL | 5.229 mL |
| 10 mM | 26.145 µL | 130.726 µL | 261.452 µL | 1.307 mL | 2.615 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Tide Quencher™ 2WS acid [TQ2WS acid] | 48000 | 0.100 | 0.120 |
| Tide Quencher™ 4WS acid [TQ4WS acid] | 900001 | 0.149 | 0.136 |
| Tide Quencher™ 5WS acid [TQ5WS acid] | 130000 | 0.072 | 0.082 |
| Tide Quencher™ 6WS acid [TQ6WS acid] | 130000 | 0.120 | 0.102 |
| Tide Quencher™ 7WS acid [TQ7WS acid] | 140000 | 0.072 | 0.091 |
| Tide Quencher™ 1 acid [TQ1 acid] | 20000 | 0.147 | 0.194 |
| Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å* | 21000 | 0.100 | 0.12 |
| Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å* | 21000 | 0.100 | 0.12 |
| Tide Quencher™ 2 phosphoramidite [TQ2 phosphoramidite] | 21000 | 0.100 | 0.12 |
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Citations
View all 8 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Polypeptide substrate for the detection of von williebrand factor cleaving protease adamts13
Authors: Visentin, Gian Paolo and Chance, Suzette C and Wuitschick, Elizabeth
Journal: (2017)
Authors: Visentin, Gian Paolo and Chance, Suzette C and Wuitschick, Elizabeth
Journal: (2017)
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
References
View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
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