Tide Fluor™ 8WS amine [TF8WS amine] Near Infrared Emission

Tide Fluor™ 8WS (TF8WS) family has the spectral properties similar to those of IRDye 800. Their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family more robust to pH-sensitive assays. In some cases TF8WS-labeled peptides and nucleotides exhibit stronger fluorescence and higher photostability than the ones labeled with IRDye 800. In pairing with our Tide Quencher™ 8WS (TQ8WS), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF8WS product is primarily used for labeling the small molecules that contain a carbonyl group.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 8WS amine [TF8WS amine] *Near Infrared Emission* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	82.425 µL	412.126 µL	824.253 µL	4.121 mL	8.243 mL
5 mM	16.485 µL	82.425 µL	164.851 µL	824.253 µL	1.649 mL
10 mM	8.243 µL	41.213 µL	82.425 µL	412.126 µL	824.253 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
Tide Fluor™ 1 amine [TF1 amine] Superior replacement for EDANS	341	447	20000	0.91¹	0.187
Tide Fluor™ 2 amine [TF2 amine] Superior replacement for fluorescein	503	525	75000	0.9¹	0.09
Tide Fluor™ 3 amine [TF3 amine] Superior replacement for Cy3	553	578	75000¹	0.1¹	0.179
Tide Fluor™ 5WS amine [TF5WS amine] Superior replacement for Cy5	649	663	250000	0.27¹	0.027
Tide Fluor™ 4 amine [TF4 amine] Superior replacement for ROX and Texas Red	577	602	90000	0.77¹	0.436
Tide Fluor™ 6WS amine [TF6WS amine] Superior replacement for Cy5.5	682	701	220000	0.27¹	0.101
Tide Fluor™ 7WS amine [TF7WS amine] Superior replacement for Cy7	756	780	275000	0.3¹	0.049
Tide Fluor™ 3WS amine [TF3WS amine] Superior replacement for Cy3	550	563	150000	0.15¹	0.079
Tide Fluor™ 2WS Amine [TF2WS amine] Superior replacement for FITC	491	516	75000	0.9¹	0.11

Citations

View all 8 citations: Citation Explorer

Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular Imaging and Biology (2023): 1--14

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16

References

View all 25 references: Citation Explorer

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311

Page updated on November 21, 2024

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Catalog Number	2336
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Physical properties

Molecular weight	1213.22
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.109
Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	785
Emission (nm)	801
Quantum yield	0.21¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501